Abstract

Grain legume crops are rich in nutritional value and play a crucial role in global food sustainability. Like many other crops, they are affected by various abiotic stresses that reduce yield and seed quality, thereby threatening food security. Several strategies have been proposed to mitigate these effects and enhance yield. Among them, the use of biostimulants offers a sustainable and efficient approach to improving stress tolerance in the short term. However, the molecular mechanisms underlying the effects of individual or combined molecules remain poorly understood and could significantly influence the development of edited crops with enhanced stress tolerance in the long term. Melatonin (MT) has emerged as a versatile biostimulant, providing multiple benefits across different crop species. Given its key role in plant physiological processes, along with endogenous production, receptor identification, and signaling functions, it has been suggested to act as a hormone-like molecule. Nonetheless, the molecular response triggered by its application remains under investigation, particularly in grain legume species. This review explores the current state of MT applications for alleviating abiotic stress in grain legume crops, with emphasis on drought, salinity, metals/metalloids, and heat stress. We integrate biochemical, molecular, and physiological evidence to highlight the main scientific gaps regarding MT function in grain legumes. Finally, we discuss the biotechnological prospects of combining MT with modern breeding tools, as well as strategies for its delivery and sustainable production. © 2025 by the authors.