Abstract

The authors regret that there are some errors in the description of the “2.10 Positron emission tomography with 2-deoxy-2-[fluorine-18]fluoro-D-glucose (18F-FDG PET)” section. The results and analysis of the our data remain correct and unchanged. The correct description of the 2.10 section is detailed as follow: “To evaluate BAT activity, 18F-FDG PET images were acquired at 14 weeks of age using a small-animal PET Scanner (Triumph™ – Gamma Medica-Ideas, Northridge, CA, U.S.A). Briefly, animals were housed 24 h before PET scans at the facility of Nuclear Medicine Laboratory (LIM-43), University of São Paulo, São Paulo, Brazil. The bolus of 37–55 MBq of 18F-FDG was administered in the penile vein under adrenergic stimulation or basal conditions. Under stimulated conditions, an intravenous injection of CL316,243 (1 mg/kg) was given 30 min before 18F-FDG administration. In the basal state, animals received an intravenous saline injection. Images were acquired 45 min after 18F-FDG injection, with the animal under isoflurane inhalation anesthesia (1.5–3%). Emission sinograms were iteratively reconstructed with the algorithm OSEM 3D; 20 iterations and 4 subsets, after being normalized and corrected for scatter and radioactivity decay. Polygonal regions of interest were drawn on sagittal PET slices for interscapular BAT. Circular regions of interest were placed in the left lung as background activity. From each region of interest, mean standardized uptake values (SUVs mean) and target-to-background ratios were measured as indices of 18F-FDG uptake. PhD. Caroline Cristiano Real Gregório and PhD. Daniele de Paula Faria, from the Laboratory of Nuclear Medicine of the University of Sao Paulo (LIM-43), Sao Paulo, Brazil, revised the description of the methodology for PET-CT protocol, performed the experiments related to this protocol, and helped with the data interpretation and presentation of the results from Fig. 10”. The authors would like to apologise for any inconvenience caused.