Generation and evaluation of a monoclonal antibody anti-pili K99 from enteropathogenic Escherichia coli Generación y evaluación de un anticuerpo monoclonal anti-pili K99 de Escherichia coli enteropatógena

Cortes C.; Vallejos P.; Bustos C.; Molina, M. C.; Aguillon, J.C.; Ferreira A.; Zurita, L

Abstract

From a culture of Escherichia coli, strain B44, pili K99 was purified and used for generating murine monoclonal antibodies. These IgG1 antibodies, purified from ascitis by immunoaffinity Protein G-Sepharose chromatography, maintained their biological activity in immunoradiometric assays. Since enterotoxigenic E. coli is one of the main agents of neonatal bovine diarrhoea, it is biotechnologically important to generate monoclonal antibodies that recognize one of the main adherence factors, pili K99. Thus, these antibodies offer an interesting possibility to detect the bacteria in biological fluids and to attempt the immunoaffinity purification of the adherence factor.

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Título de la Revista: ARCHIVOS DE MEDICINA VETERINARIA
Volumen: 28
Número: 2
Editorial: UNIV AUSTRAL CHILE, FAC CIENCIAS VETERINARIAS
Fecha de publicación: 1996
Página de inicio: 137
Página final: 141
URL: http://www.scopus.com/inward/record.url?eid=2-s2.0-0030376950&partnerID=q2rCbXpz