GTX 2/3 epimers permeate the intestine through a paracellular pathway

Torres R.; Pizarro, L; Garcia C.; Lagos, N.; Csendes, A

Keywords: kinetics, model, diffusion, permeability, junction, culture, absorption, impedance, humans, human, time, intestine, epithelium, tissue, saxitoxin, drug, mucosa, article, isomerism, dose-response, cation, jejunum, junctions, concentration, gonyautoxin, controlled, technique, study, Relationship,, biological, Models,, Electric, unclassified, Intestinal, ii, tight, (parameters), iii, epimer, Chambers,


The aim of this work was to typify the mechanisms involved in gonyautoxins intestinal permeability. For this purpose, permeability of gonyautoxins through intestinal epithelium, and their effect on transepithelial resistance was investigated in excised human jejunal segments. The isolated mucosa segments were mounted in a Ussing chamber and experiments performed under voltage-controlled conditions. Organic gonyautoxin cations were applied in the apical side and samples collected in the basolateral side. Results show that gonyautoxin 2/3 epimers (GTX 2/3) permeate the intestine through a paracellular pathway and, to reach the resolution of the technique we used, no evidence was found of any other transport mechanism involved in the process. A model was developed, according to which tight junctions undergo a toxin concentration and time-dependent change, while transepithelial resistance shows a modest decrease.

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Título según SCOPUS: GTX 2/3 epimers permeate the intestine through a paracellular pathway
Título de la Revista: Journal of Toxicological Sciences
Volumen: 32
Número: 3
Editorial: Japanese Society of Toxicological Sciences
Fecha de publicación: 2007
Página de inicio: 241
Página final: 248