Delphinidin Activates NFAT and Induces IL-2 Production Through SOCE in T Cells
Delphinidin is an anthocyanidin that possesses antioxidant and anti-inflammatory effects; however, some reports suggest that delphinidin has pro-inflammatory properties. For this reason, we assessed the effect of delphinidin on cytokine production in T cells. We demonstrated that delphinidin increased the cytosolic-free Ca2+ concentration by releasing Ca2+ from intracellular stores and increasing Ca2+ entry. The putative Ca2+ release activated Ca2+ (CRAC) channel inhibitors BTP2 and gadolinium reduced the calcium entry stimulated by the anthocyanidin. Delphinidin induced nuclear factor of activated T cells (NFAT) translocation and NFAT-Luc activity in Jurkat cells and was dependent on the CRAC channel and calcineurin pathway. Delphinidin increased the mRNA expression and production of IL-2 in Jurkat cells and was inhibited by BTP2 and cyclosporine A. Using peripheral blood lymphocytes, we demonstrated that delphinidin increased the production of IL-2 and IFN-gamma and was inhibited by BTP2. Taken together, our results suggest that delphinidin exerts immunostimulatory effects on T cells by increasing cytokine production through CRAC channel and NFAT activation.
|Título según WOS:||Delphinidin Activates NFAT and Induces IL-2 Production Through SOCE in T Cells|
|Título según SCOPUS:||Delphinidin Activates NFAT and Induces IL-2 Production Through SOCE in T Cells|
|Título de la Revista:||CELL BIOCHEMISTRY AND BIOPHYSICS|
|Editorial:||Humana Press, Inc.|
|Fecha de publicación:||2014|
|Página de inicio:||497|