Combined microbiological test to assess changes in an organic matrix used to avoid agricultural soil contamination, exposed to an insecticide

Tortella, G.R; Salgado E.; Cuozzo, S.A.; Mella-Herrera R.A.; Parra, L; Diez, M.C.; Rubilar, O

Keywords: Biopurification system; CLFP; Diazinon; Microbial communities

Abstract

Combined microbiological and molecular test (BiologEcoplateTM , denaturing gradient gel electrophoresis (DGGE) and Real Time PCR (qPCR)) were carried out to evaluate the impact of repeated diazinon (DZN) applications at high concentration (40 mg kg-1) on microbial communities in a microcosm simulating the organic matrix (straw (50%): peat (25%): soil (25%) vv-1) of a pesticide biopurification system (PBS). Pesticide dissipation was also evaluated. After three successive exposures with DZN the dissipation efficiency was high; DZN dissipation clearly accelerated in the organic matrix, achieving 87%, 93% and 96% respectively after the three applications. The results obtained with BiologEcoplate TM showed that the physiological profiles of the community were not affected by the addition of DZN. On the other hand, molecular assays (DGGE and qPCR) demonstrated that the microbial structure (bacteria and fungi) remained relatively stable over time with high DZN doses compared to control. The results of the present study clearly demonstrate the high dissipation capacity of this biomixture and highlight the microbiological robustness of this biological system.

Más información

Título según SCOPUS: Combined microbiological test to assess changes in an organic matrix used to avoid agricultural soil contamination, exposed to an insecticide
Título según SCIELO: Combined microbiological test to assess changes in an organic matrix used to avoid agricultural soil contamination, exposed to an insecticide
Título de la Revista: Journal of Soil Science and Plant Nutrition
Volumen: 14
Número: 4
Editorial: Sociedad Chilena de la Ciencia del Suelo
Fecha de publicación: 2014
Página de inicio: 869
Página final: 880
Idioma: English
DOI:

10.4067/S0718-95162014000400009

Notas: SCIELO, SCOPUS