Acetylcholine sensitivity in sensory neurons dissociated from the cat petrosal ganglion

Varas, R.; Alcayaga J.; Zapata, P

Abstract

The petrosal ganglia contain the somata of the sensory fibers of the glossopharyngeal nerves, innervating structures of the tongue, pharynx, carotid sinus and carotid body. Petrosal ganglia were excised from adult cats and their neurons were dissociated and kept in tissue culture for 7-12 days. Intracellular recordings were obtained through conventional microelectrodes. In response to depolarizing pulses, most cells (41/60) presented a 'hump' in the falling phase of their action potentials (H-type), while the remaining neurons lack such hump (F-type). The two types of cells had no differences in resting membrane potential or action potential amplitude. Acetylcholine (ACh) applied locally elicited responses in nearly two thirds of both H-type and F-type neurons tested. Most H-type neurons (17/19) responded with a slow long lasting depolarization, while the remaining (2) did so by generating spikes. In contrast, half of F-type neurons (6/12) responded with one or more spikes and the other half only with a slow depolarization. These results indicate that ACh receptors are present in the soma of many petrosal ganglion neurons subjected to tissue culture, thus supporting the idea that - under normal conditions - their peripheral sensory processes may be excited by ACh. (C) 2000 Elsevier Science B.V. All rights reserved.

Más información

Título según WOS: Acetylcholine sensitivity in sensory neurons dissociated from the cat petrosal ganglion
Título según SCOPUS: Acetylcholine sensitivity in sensory neurons dissociated from the cat petrosal ganglion
Título de la Revista: BRAIN RESEARCH
Volumen: 882
Número: 1-2
Editorial: Elsevier
Fecha de publicación: 2000
Página de inicio: 201
Página final: 205
Idioma: English
URL: http://linkinghub.elsevier.com/retrieve/pii/S0006899300028171
DOI:

10.1016/S0006-8993(00)02817-1

Notas: ISI, SCOPUS