A dorsal root ganglia cell line derived from trisomy 16 fetal mice, a model for Down syndrome

Allen, DD; Cárdenas AM; Arriagada C.; Bennett, LB; Garcia, CJ; Caviedes R.; Rapoport, SI; Caviedes P.

Abstract

We have established two immortalized cell lines from dorsal root ganglia of normal (G4b) and trisomy 16 mice (GTI), a model for Down syndrome. By immunohistochemistry, both cell lines exhibit neuronal traits and lack glial markers. GTI cells exhibited greater [3H]choline uptake than G4b cells. K+ and nicotine-mediated acetylcholine release was greater in GTI cells. Basal intracellular Ca2+ concentration ([Ca2+]i) was significantly lower in GTI cells. More GTI cells responded to neurotransmitters with a transient [Ca2+]i increase compared to G4b cells, but both cell types showed similar amplitudes of [Ca2+]i responses. The results show that both cell lines retain neuronal characteristics and respond to specific neurotransmitter stimuli. Altered GTI cell responses could be related to neuronal pathophysiology in Down's syndrome. © 2002 Lippincott Williams & Wilkins.

Más información

Título según WOS: A dorsal root ganglia cell line derived from trisomy 16 fetal mice, a model for Down syndrome
Título según SCOPUS: A dorsal root ganglia cell line derived from trisomy 16 fetal mice, a model for Down syndrome
Título de la Revista: NEUROREPORT
Volumen: 13
Número: 4
Editorial: LIPPINCOTT WILLIAMS & WILKINS
Fecha de publicación: 2002
Página de inicio: 491
Página final: 496
Idioma: English
URL: http://content.wkhealth.com/linkback/openurl?sid=WKPTLP:landingpage&an=00001756-200203250-00027
DOI:

10.1097/00001756-200203250-00027

Notas: ISI, SCOPUS