Antioxidant Effect of a Polyphenol-Rich Murtilla (Ugni molinae Turcz.) Extract and Its Effect on the Regulation of Metabolism in Refrigerated Boar Sperm

Jofre, Ignacio; Cuevas, Magdalena; de Castro, Leticia Signori; de Agostini Losano, Joao Diego; Torres, Mariana Andrade; Alvear, Marysol; Scheuermann, Erick; Cesar Andrade, Andre Furugen; Nichi, Marcilio; Ortiz Asstumpcao, Mayra Elena; Romero, Fernando

Abstract

The production of reactive oxygen species (ROS) in boar spermatozoa increases in refrigeration; this can have an impact on sperm quality and fertilization capacity. We evaluated the effect of polyphenol-rich aqueous extract of murtilla (Ugni molinae Turcz) on boar sperm stored at 17 degrees C in order to reduce oxidative stress and improve sperm quality in the long term. Five experiments were performed: first, characterization of the polyphenol content from five genotypes of murtilla; second, determination of the genotype with the best antioxidant effect (MT-Ex); third, the antioxidant capacity on O-2(-) and lipid peroxidation; fourth, the influence of MT-Ex on motility, calcium movement, cAMP, and metabolic parameters; and fifth, analysis of long-term refrigeration. The average phenolic content was 344 ppm; gallic acid, catechin, quercetin, myricetin, and kaempferol were detected. All extracts evaluated presented a concentration-dependent antioxidant effect. MT-Ex reduces intracellular O-2(-)/peroxides but low lipid peroxidation. MT-Ex in nonstimulated ROS conditions reduces sperm motility, mitochondrial membrane potential, cAMP, and ATP, but the succinate dehydrogenase activity remained normal; also, we observed a reduction in calcium movement in in vitro sperm capacitation. The long-term analyses showed that MT-Ex improved sperm motility decay and reduced membrane damage and ROS at 168 h. Based on this study, we propose MT-Ex as a supplement in semen extenders.

Más información

Título según WOS: Antioxidant Effect of a Polyphenol-Rich Murtilla (Ugni molinae Turcz.) Extract and Its Effect on the Regulation of Metabolism in Refrigerated Boar Sperm
Título según SCOPUS: Antioxidant Effect of a Polyphenol-Rich Murtilla (Ugni molinae Turcz.) extract and its effect on the regulation of metabolism in refrigerated boar sperm
Título de la Revista: OXIDATIVE MEDICINE AND CELLULAR LONGEVITY
Volumen: 2019
Editorial: HINDAWI LTD
Fecha de publicación: 2019
Idioma: English
DOI:

10.1155/2019/2917513

Notas: ISI, SCOPUS