HIF1 alpha-dependent metabolic reprogramming governs mesenchymal stem/stromal cell immunoregulatory functions

Contreras-Lopez, Rafael; Elizondo-Vega, Roberto; Jose Paredes, Maria; Luque-Campos, Noymar; Torres, Maria Jose; Tejedor, Gautier; Maria Vega-Letter, Ana; Figueroa-Valdes, Aliosha; Pradenas, Carolina; Oyarce, Karina; Jorgensen, Christian; Khoury, Maroun; de los Angeles Garcia-Robles, Maria; Altamirano, Claudia; Djouad, Farida; et. al.

Abstract

Hypoxia-inducible factor 1 alpha (HIF1 alpha), a regulator of metabolic change, is required for the survival and differentiation potential of mesenchymal stem/stromal cells (MSC). Its role in MSC immunoregulatory activity, however, has not been completely elucidated. In the present study, we evaluate the role of HIF1 alpha on MSC immunosuppressive potential. We show that HIF1 alpha silencing in MSC decreases their inhibitory potential on Th1 and Th17 cell generation and limits their capacity to generate regulatory T cells. This reduced immunosuppressive potential of MSC is associated with a metabolic switch from glycolysis to OXPHOS and a reduced capacity to express or produce some immunosuppressive mediators including Intercellular Adhesion Molecule (ICAM), IL-6, and nitric oxide (NO). Moreover, using the Delayed-Type Hypersensitivity murine model (DTH), we confirm, in vivo, the critical role of HIF1 alpha on MSC immunosuppressive effect. Indeed, we show that HIF1 alpha silencing impairs MSC capacity to reduce inflammation and inhibit the generation of pro-inflammatory T cells. This study reveals the pivotal role of HIF1 alpha on MSC immunosuppressive activity through the regulation of their metabolic status and identifies HIF1 alpha as a novel mediator of MSC immunotherapeutic potential.

Más información

Título según WOS: ID WOS:000529448500001 Not found in local WOS DB
Título de la Revista: FASEB JOURNAL
Editorial: Wiley
Fecha de publicación: 2020
DOI:

10.1096/fj.201902232R

Notas: ISI