A Pyranine based Procedure for Evaluation of the Total Antioxidant Potential (TRAP) of Polyphenols: A Comparison with closely related Methodologies

Campos, AM; Sotomayor, CP; Pino E.; Lissi E.

Abstract

A novel procedure for the evaluation of total reactive antioxidant potentials (TRAP) is described. The method is based on the measurement of the bleaching of pyranine by peroxyl radicals. The addition of the antioxidants produces a clear induction time whose magnitude is directly related to the antioxidant concentration. A comparison of the values obtained with those reported employing closely related methodologies shows that the results are significantly affected by the substrate employed to monitor the steady state free radical concentration. Possible sources of this dependence are discussed

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Título según WOS: A pyranine based procedure for evaluation of the total antioxidant potential (TRAP) of polyphenols. A comparison with closely related methodologies
Título según SCOPUS: A pyranine based procedure for evaluation of the total antioxidant potential (TRAP) of polyphenols. A comparison with closely related methodologies
Título según SCIELO: A Pyranine based Procedure for Evaluation of the Total Antioxidant Potential (TRAP) of Polyphenols: A Comparison with closely related Methodologies
Título de la Revista: Biological Research
Volumen: 37
Número: 2
Editorial: Sociedad de Biología de Chile
Fecha de publicación: 2004
Página de inicio: 287
Página final: 292
Idioma: English
DOI:

10.4067/S0716-97602004000200015

Notas: ISI, SCIELO, SCOPUS