The ependymocytes of the bovine subcommissural organ are functionally coupled through gap junctions

Gonzalez, CA; Garces, G; Saez, JC; Schobitz, K; Rodriguez, EM


The subcommissural organ (SCO) is a circunventricular organ secreting glycoproteins into the ventricle. It is richly innervated by (1) serotonergic fibers originated in raphe nuclei, that would exert an inhibitory control, and (2) peptidergic fibers of unknown function. Due to the scarce number of the latter, their functional significance might largely depends on whether the cells of the SCO are functionally coupled through gap junctions. This investigation was designed to answer this question. The bovine SCO, either freshly isolated or maintained in organ culture, was processed for immunoblot and immunocytochemistry, using an anti-connexin43 antibody, and dye coupling studies. It was found that the cells of the SCO in situ are functionally coupled through gap junctions made at least of connexin43, but in cultured explants are not. The possibility that coupling of the SCO may be controlled by the neural input and undergoes circadian variations is discussed. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.

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Título según WOS: ID WOS:000079171400008 Not found in local WOS DB
Título de la Revista: NEUROSCIENCE LETTERS
Volumen: 262
Número: 3
Fecha de publicación: 1999
Página de inicio: 175
Página final: 178


Notas: ISI