Development and Characterization of Microsatellite Loci in Gaultheria pumila Lf. (Ericaceae)

Rolando Garcia-Gonzales; José Pico-Mendoza; Karla Quiroz; Basilio Carrasco; Pablo Cáceres; Borys Chong-Perez; Hugo Pino; Marjorie Seiltgens; Eglis Greck; Peter D. S. Caligari

Keywords: Microsatellite, Pyrosequencing, Chilean berry, Gaultheria pumila


Polymorphic microsatellite markers were developed for Gaultheria pumila (Ericaceae) to evaluate genetic diversity and population structure within its native range in Chile. This is a very important Ericaceae endemic to Chile with a large commercial potential. Its resistance to diferent abiotic conditions makes it a valuable target for genetic improvement.Ten polymorphic simple sequence repeat (SSR) loci were isolated from Gaultheria pumila using newgeneration 454 FLX Titanium pyrosequencing technology. The mean number of alleles per locus ranged from 2 to 4. Observed and expected heterozygosity ranged from 0.00 to 1.0 and 0.00 to 0.64, respectively. From 10 SSR markers developed for G. pumila, 9 markers are promising candidates for analyzing genetic variation within or between natural populations of G. pumila and other species from the same genus.

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Título de la Revista: Biological Research volume
Volumen: 51
Editorial: Springer
Fecha de publicación: 2018
Página de inicio: 42
Página final: 45
Idioma: English


Notas: WOS core collection ISI