VALIDATION OF A METHOD TO DETECT CENTRAL NERVOUS SYSTEM ACTING DRUGS IN BLOOD BY GC/MS. APPLICATION IN CASES OF DEATH CAUSED BY PHENOTHIAZINES IN THE NORTH OF CHILE

Felipe Bravo; Roberto Ulloa; KARINA VENEGAS; CONTZEN, MARÍA CECILIA; DAVID RÍOS; BriceÑo, Margarita A.; PEDRO BUC CALDERONI; Benites, Julio

Keywords: antiepileptic and antipsychotics drugs, gas chromatography, mass selective detector, solid-phase extraction

Abstract

A simple and rapid analytical method is developed and validated for the determination of antiepileptic and antipsychotic drugs in human whole blood using a solid-phase extraction and quantification by gas chromatography–mass spectrometry (GC/MS). Prazepam was used as internal standard (IS). The specificity, linearity, intra- and inter-assay precision and accuracy, and extraction recovery were fully evaluated. The limits of detection and quantification were in the range of 0.52 - 0.98 mg/mL and 1.56 - 2.97 mg/mL for antiepileptic drugs (pentobarbital, phenobarbital and carbamazepine) and 1.31 - 2.94 ng/mL and 3.98 - 8.89 ng/mL for antipsychotic drugs (chlorpromazine and thioridazine), respectively. The relative standard deviation (RSD) was less than 7.07%, while the intra-day accuracy was < 3.21% and the inter-day was < 8.80%, referred to RSD. The developed methodology was applied in forensic cases of death caused by phenothiazines in the North of Chile during 2010-2012.

Más información

Título de la Revista: Journal of the Chilean Chemical Society
Volumen: 59(3)
Editorial: 2013
Fecha de publicación: 2014
Página de inicio: 2602
Página final: 2605
Idioma: Inglés
URL: https://scielo.conicyt.cl/scielo.php?pid=S0717-97072014000300016&script=sci_arttext#a1