Antiplatelet activity of Isorhamnetin via mitochondrial regulation

Rodríguez L.; Badimon L.; Méndez D.; Padró T.; Vilahur G.; Peña E.; Carrasco B.; Vogel H.; Palomo I.; Fuentes E.

Keywords: flavonoids, foods, isorhamnetin, antiplatelet, antithrombotic


With the diet, we ingest nutrients capable of modulating platelet function, which plays a crucial role in developing cardiovascular events, one of the leading causes of mortality worldwide. Studies that demonstrate the antiplatelet and antithrombotic potential of bioactive compounds are vital to maintaining good cardiovascular health. In this work, we evaluate the flavonol isorhamnetin’s antiplatelet effect on human platelets, using collagen, thrombin receptor activator peptide 6 (TRAP-6), and phorbol myristate acetate (PMA) as agonists. Isorhamnetin induced a significant inhibition on collagen- and TRAP-6-induced platelet aggregation, with half-maximum inhibitory concentration (IC50) values of 8.1 ± 2.6 and 16.1 ± 11.1 µM, respectively; while it did not show cytotoxic effect. Isorhamnetin reduced adenosine triphosphate levels (ATP) in platelets stimulated by collagen and TRAP-6. We also evidenced that isorhamnetin’s antiplatelet activity was related to the inhibition of mitochondrial function without effect on reactive oxygen species (ROS) levels. Additionally, we investigated isorhamnetin’s effect on thrombus formation in vitro under flow conditions on the damaged vessel wall. In this context, we demonstrate that isorhamnetin at 20 µM induced a significant inhibition on platelet deposition, confirming its antithrombotic effect. Our findings corroborate the antiplatelet and antithrombotic potential of isorhamnetin present in many foods of daily consumption

Más información

Título de la Revista: Antioxidants
Volumen: 10
Fecha de publicación: 2021
Página de inicio: 666
Página final: 677
Idioma: Inglés
Financiamiento/Sponsor: This research was funded by Centro de Estudios en Alimentos Procesados-CEAP, ANID, Programa Regional R20F0001, Talca, Chile.