BINDING OF ROSE BENGAL TO BOVINE SERUM ALBUMIN

Abuin E.; Aspée A.; Lissi E.; Leon, L.

Abstract

The association of Rose Bengal (RB) with bovine serum albumin (BSA) was investigated by absorbance spectroscopy. The binding constant was determined from the effect observed in the absorbance of RB at 548 nm upon addition of the protein according with the Benesi-Hildebrand treatment. Results were obtained in phosphate buffer at pH = 7.0. The effect of the salinity of the buffer and the sensitivity of the binding constant to the presence of urea were also studied. The results obtained allow to conclude that the binding of RB to BSA is dominated by hydrophobic effects

Más información

Título según WOS: Binding of rose bengal to bovine serum albumin
Título según SCOPUS: Binding of Rose Bengal to bovine serum albumin
Título según SCIELO: BINDING OF ROSE BENGAL TO BOVINE SERUM ALBUMIN
Título de la Revista: Journal of the Chilean Chemical Society
Volumen: 52
Número: 2
Editorial: 2013
Fecha de publicación: 2007
Página de inicio: 1196
Página final: 1197
Idioma: English
DOI:

10.4067/S0717-97072007000200017

Notas: ISI, SCIELO, SCOPUS