Physodic acid sensitizes LNCaP prostate cancer cells to TRAIL-induced apoptosis

Cardile, Venera; Graziano, Adriana C. E.; Avola, Rosanna; Madrid, Alejandro; Russo, Alessandra

Abstract

In spite of the extensive research for developing new therapies, prostate cancer is still one of the major human diseases with poor prognosis and high mortality. Therefore, with the aim of identifying novel agents with antigrowth and pro-apoptotic activity on prostate cancer cells, in the present study, we evaluated the effect of lichen secondary metabolite physodic acid on cell growth in human prostate cancer cells. In addition, we tested the apoptotic activity of physodic acid on TRAIL-resistant LNCaP cells in combination with TRAIL. The cell viability was measured using MTT assay. LDH release, a marker of membrane breakdown, was also measured. For the detection of apoptosis, the evaluation of DNA fragmentation and caspase-3 activity assay were employed. The expression of proteins was detected by Western blot analysis. It was observed that physodic acid showed a dose-response relationship in the range of 12.5-50 mu M concentrations in LNCaP and DU-145 cells, activating an apoptotic process. In addition, physodic acid sensitizes LNCaP cells to TRAIL-induced apoptosis. The combination of physodic acid with other anti-prostate cancer therapies could be considered a promising strategy that warrants further investigations.

Más información

Título según WOS: ID WOS:000913784400006 Not found in local WOS DB
Título de la Revista: Toxicology in vitro : an international journal published in association with BIBRA.
Volumen: 84
Editorial: PERGAMON-ELSEVIER SCIENCE LTD
Fecha de publicación: 2022
DOI:

10.1016/j.tiv.2022.105432

Notas: ISI