Production of (R)-3-hydroxybutyric acid from methane by in vivo depolymerization of polyhydroxybutyrate in Methylocystis parvus OBBP

Yanez, Luz; Rodriguez, Yadira; Scott, Felipe; Vergara-Fernandez, Alberto; Munoz, Raul

Abstract

Methylocystis parvus OBBP accumulates polyhydroxybutyrate (PHB) using methane as the sole carbon and energy source. In this work, the feasibility of producing (R)-3-hydroxybutyric acid (R3HBA) via intracellularly accumulated PHB through depolymerization (in-vivo) was investigated. Results showed that a PHB to R3HBA conversion of 77.2 +/- 0.9% (R3HBA titer of 0.153 +/- 0.002 g L-1) can be attained in a mineral medium containing 1 g L-1 KNO3 at 30 degrees C with shaking at 200 rpm and a constant pH of 11 for 72 h. Nitrogen deprivation and neutral or acidic pHs strongly reduced the excreted R3HBA concentration. Reduced oxygen availability negatively affected the R3HBA yield, which decreased to 73.6 +/- 4.9% (titer of 0.139 +/- 0.01 g L-1) under microaerobic conditions. Likewise, the presence of increasing concentrations of R3HBA in the medium before the onset of PHB depolymerization reduced the initial R3HBA release rate and R3HBA yield.

Más información

Título según WOS: ID WOS:000795664400012 Not found in local WOS DB
Título de la Revista: BIORESOURCE TECHNOLOGY
Volumen: 353
Editorial: ELSEVIER SCI LTD
Fecha de publicación: 2022
DOI:

10.1016/j.biortech.2022.127141

Notas: ISI