Direct Thy-1/alpha(V)beta(3) integrin interaction mediates neuron to astrocyte communication

Hermosilla T.; Muñoz D; Herrera-Molina, R; Valdivia, A; Muñoz, N.; Nham, SU; Schneider, P.; Burridge, K; Quest, AFG; Leyton, L

Abstract

Thy-1 is an abundant neuronal glycoprotein of poorly defined function. We recently provided evidence indicating that Thy-1 clusters a β3-containing integrin in astrocytes to induce tyrosine phosphorylation, RhoA activation and the formation of focal adhesions and stress fibers. To date, the α subunit partner of β3 integrin in DI TNC1 astrocytes is unknown. Similarly, the ability of neuronal, membrane-bound Thy-1 to trigger astrocyte signaling via integrin engagement remains speculation. Here, evidence that αv forms an αvβ3 heterodimer in DI TNC1 astrocytes was obtained. In neuron-astrocyte association assays, the presence of either anti-αv or anti-β3 integrin antibodies reduced cell-cell interaction demonstrating the requirement of both integrin subunits for this association. Moreover, anti-Thy-1 antibodies blocked stimulation of astrocytes by neurons but not the binding of these two cell types. Thus, neuron-astrocyte association involved binding between molecular components in addition to the Thy-1-integrin; however, the signaling events leading to focal adhesion formation in astrocytes depended exclusively on the latter interaction. Additionally, wild-type (RLD) but not mutated (RLE) Thy-1 was shown to directly interact with αvβ3 integrin by Surface Plasmon Resonance analysis. This interaction was promoted by divalent cations and was species-independent. Together, these results demonstrate that the αvβ3 integrin heterodimer interacts directly with Thy-1 present on neuronal cells to stimulate astrocytes. © 2008 Elsevier B.V. All rights reserved.

Más información

Título según WOS: Direct Thy-1/alpha(V)beta(3) integrin interaction mediates neuron to astrocyte communication
Título según SCOPUS: Direct Thy-1/aVß3 integrin interaction mediates neuron to astrocyte communication
Título de la Revista: Biochim Biophys Acta Mol Cell Res
Volumen: 1783
Número: 6
Editorial: Elsevier
Fecha de publicación: 2008
Página de inicio: 1111
Página final: 1120
Idioma: English
URL: http://linkinghub.elsevier.com/retrieve/pii/S0167488908000530
DOI:

10.1016/j.bbamcr.2008.01.034

Notas: ISI, SCOPUS