Direct Thy-1/alpha(V)beta(3) integrin interaction mediates neuron to astrocyte communication
Thy-1 is an abundant neuronal glycoprotein of poorly defined function. We recently provided evidence indicating that Thy-1 clusters a Î²3-containing integrin in astrocytes to induce tyrosine phosphorylation, RhoA activation and the formation of focal adhesions and stress fibers. To date, the Î± subunit partner of Î²3 integrin in DI TNC1 astrocytes is unknown. Similarly, the ability of neuronal, membrane-bound Thy-1 to trigger astrocyte signaling via integrin engagement remains speculation. Here, evidence that Î±v forms an Î±vÎ²3 heterodimer in DI TNC1 astrocytes was obtained. In neuron-astrocyte association assays, the presence of either anti-Î±v or anti-Î²3 integrin antibodies reduced cell-cell interaction demonstrating the requirement of both integrin subunits for this association. Moreover, anti-Thy-1 antibodies blocked stimulation of astrocytes by neurons but not the binding of these two cell types. Thus, neuron-astrocyte association involved binding between molecular components in addition to the Thy-1-integrin; however, the signaling events leading to focal adhesion formation in astrocytes depended exclusively on the latter interaction. Additionally, wild-type (RLD) but not mutated (RLE) Thy-1 was shown to directly interact with Î±vÎ²3 integrin by Surface Plasmon Resonance analysis. This interaction was promoted by divalent cations and was species-independent. Together, these results demonstrate that the Î±vÎ²3 integrin heterodimer interacts directly with Thy-1 present on neuronal cells to stimulate astrocytes. Â© 2008 Elsevier B.V. All rights reserved.
|Título según WOS:||Direct Thy-1/alpha(V)beta(3) integrin interaction mediates neuron to astrocyte communication|
|Título según SCOPUS:||Direct Thy-1/aVß3 integrin interaction mediates neuron to astrocyte communication|
|Título de la Revista:||Biochim Biophys Acta Mol Cell Res|
|Fecha de publicación:||2008|
|Página de inicio:||1111|