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Luis Alberto Mercado Vianco

Full Professor

PONTIFICIA UNIVERSIDAD CATÓLICA DE VALPARAÍSO

Valparaíso, Chile

Fish Immunology; Immunological Markers; Aquatic Organisms; Antimicrobial Peptides

Biology, PONTIFICIA UNIVERSIDAD CATOLICA DE VALPARAISO. Chile, 1991
Microbiological Sciences, Pontificia Universidad Católica de Valparaíso. Chile, 1997
Biochemistry and Molecular Biology, Universidad de Santiago de Compostela. España, 2002

Full Professor Full Time

PONTIFICIA UNIVERSIDAD CATOLICA DE VALPARAISO

Faculty of Sciences

Valparaíso, Chile

2006 - A la fecha

Postdoctoral Position Full Time

Pontificia Universidad Católica de Valparaíso

Valparaíso, Chile

2002 - 2005

Tesis dirigidas:

Graduate (PhD)

Autor(es):Claudia Palacios Jiménez.
Título: "Immune detection of lipocortin 1 in leukocytes of O.mykiss: A potential marker of immune suppression under stress conditions in teleost fish. ",
Institución: PONTIFICIA UNIVERSIDAD CATOLICA DE VALPARAISO, Doctorado/PhD, 2013

Autor(es):Roxana Gonzalez Stegmaier
Título: "Design of peptides derived from flagellin as effectors of immune stimulation through TLR5 in salmonids, and their evaluation by cellular activation and cytokine expression",
Institución: PONTIFICIA UNIVERSIDAD CATOLICA DE VALPARAISO, Doctorado/PhD, 2013

Autor(es):Paula Santana Sepulveda.
Título: "Design, generation and evaluation of a biotechnological tool for the quantification of innate immunity markers in salmonid fry. ",
Institución: PONTIFICIA UNIVERSIDAD CATOLICA DE VALPARAISO, Doctorado/PhD, 2014

Graduate (Master)

Autor(es): Sebastián Sariego B.
Título: "Modelo molecular en tejido branquial de trucha inmunoestimulado con el beta-glucano Zymosán liberado en dieta",
Institución: Universidad Andrés Bello. Magíster en Biotecnología. 2014.

Autor(es):Byron Morales
Título: "Disponibilidad de proteínas tipo peroxirredoxina en el erizo marino antártico frente a la inducción con patrones moleculares asociados a patógenos",
Institución: Pontificia Universidad Católica de Valparaíso, Magíster en Ciencias Microbiológicas. 2014.

Undergraduate

Autor(es):Cristian Valenzuela Ibarra.
Título: "Anti-epítopes de óxido nítrico sintasa inducible (iNOS) de salmónidos: Una herramienta para la evaluación de la respuesta inmune innata en peces.",
Institución: PONTIFICIA UNIVERSIDAD CATOLICA DE VALPARAISO, Licenciatura/Bachillerato/Bachelor, 2012

Autor(es):Andrés Aquiles Abarca Lange.
Título: "Implementación de un modelo in vitro para determinar el poder inmunoestimulante de β-glucanos en macrófagos de trucha arco iris (Onchorrincus mykiss)",
Institución: PONTIFICIA UNIVERSIDAD CATOLICA DE VALPARAISO, Licenciatura/Bachillerato/Bachelor, 2012

Autor(es):Byron Morales Lange.
Título: "Evaluación del efecto de laminarán sobre la inmunidad innata de Oncorhynchus mykiss.",
Institución: PONTIFICIA UNIVERSIDAD CATOLICA DE VALPARAISO, Licenciatura/Bachillerato/Bachelor, 2011

Autor(es):FERNANDO ESTEBAN ALVAREZ VEGA.
Título: "PROPUESTA DE UN MÉTODO MEJORADO PARA LA CUANTIFICACIÓN POR ELISA DE LAS CITOQUINAS PRO-INFLAMATORIAS TNF α ALPHA E IL-1β DE SALMÓNIDOS.",
Institución: UNIVERSIDAD DE CONCEPCION, Licenciatura/Bachillerato/Bachelor, 2010

Autor(es):Jörn Bethke Riegel.
Título: "Expresión de Natural Killer Enhancing Factor (NKEF)- like inducida in vitro y a nivel sistémico en respuesta a Caligus rogercresseyi en Salmónidos",
Institución: PONTIFICIA UNIVERSIDAD CATOLICA DE VALPARAISO, Licenciatura/Bachillerato/Bachelor, 2010

Autor(es):Edgar Narváez Barrera
Título: "Estrategias para la identificación de diferencias individuales en la expresión de péptidos antimicrobianos en Salmo salar (Linneaeus, 1758)",
Institución: PONTIFICIA UNIVERSIDAD CATOLICA DE VALPARAISO, Licenciatura/Bachillerato/Bachelor, 2009

Autor(es):Jorge Berendsen Mella
Título: "Desarrollo de cultivos celulares primarios de hemocitos de Choromytilus chorus (Molina, 1782)(Mollusca: Bivalvia) para la inducción in Vitro de la secreción de péptidos antimicrobianos",
Institución: PONTIFICIA UNIVERSIDAD CATOLICA DE VALPARAISO, Licenciatura/Bachillerato/Bachelor, 2007

Autor(es):Paola Matallana, Joyce Maturana y Rodrigo Reyes
Título: "Estudio exploratorio de carencias en la enseñanza de la biología celular: Propuesta de material de apoyo digital. ",
Institución: PONTIFICIA UNIVERSIDAD CATOLICA DE VALPARAISO, Licenciatura/Bachillerato/Bachelor, 2007

Autor(es):Björn Erkens
Título: "Partial purification and descripition of antimicrobial peptides in the bronchial tissue of Protothaca thaca",
Institución: Fachhochschule Bonn-Rhein-Sieg (Universidad de Ciencias Aplicadas Bonn-Rhein-Sieg), Licenciatura/Bachillerato/Bachelor, 2006

Mejor Graduado Licenciatura en Biología

PONTIFICIA UNIVERSIDAD CATOLICA DE VALPARAISO

Chile, 1991

Ranking 1 Bachellor
Mejor Graduado Magíster Ciencias Microbiológicas

Pontificia Universidad Católica de Valparaíso

Chile, 1997

Ranking 1 Master


Insight into the messenger role of reactive oxygen intermediates in immunostimulated hemocytes from the scallop Argopecten purpuratus. Oyanedel, Daniel; Gonzalez, Roxana; Brokordt, Katherina; Schmitt, Paulina; Mercado, Luis Article Developmental and comparative immunology (2016)
Pentavalent antimony uptake pathway through erythrocyte membranes: molecular and atomic fluorescence approaches Camila Barrera; Silvana López; Luis Aguilar; Luis Mercado; Manuel Bravo & Waldo Quiroz Article ANALYTICAL AND BIOANALYTICAL CHEMISTRY (2016)
Synthetic hepcidin from fish: Uptake and protection against Vibrio anguillarum in sea bass (Dicentrarchus labrax) Alvarez, CA; F. Acosta; D. Montero; F. Guzmán; E. Torres; B.Vega L. Mercado Article Fish and Shellfish Immunology (2016)
Immune response of the Antarctic sea urchin Sterechinus neumayeri: cellular, molecular and physiological approach Marcelo Gonzalez-Aravena; C. Perez-Troncoso; R. Urtubia; P. Branco; J.R. Machado Cunha da Silva; Luis Mercado ,Julien De Lorgeril; J. Bethke & K. Paschke Article Revista de Biología Tropical (2015)
Sb(V) Reactivity with Human Blood Components: Redox Effects López S.; Aguilar L.; Mercado L.; Bravo M.; Quiroz W. Article PLOS ONE (2015)
Antimicrobial activity of trout hepcidin Alvarez CA, Guzmán F, Cárdenas C, Marshall SH, Mercado L. Article ISI SCOPUS Fish & Shellfish Immunology (2014)
Antimicrobial activity of trout hepcidin Alvarez CA, Guzmán F, Cárdenas C, Marshall SH, Mercado L. Article Fish and Shellfish Immunology (2014)
Phenotypical parameters as a tool to evaluate the immunostimulatory effects of laminarin in Oncorhynchus mykiss Morales-Lange, Byron; Bethke, Jörn; Schmitt, Paulina; Mercado, Luis Article AQUACULTURE RESEARCH (2014)
Pro-inflammatory caspase-1 activation during the immune response in cells from rainbow trout Oncorhynchus mykiss (Walbaum 1792) challenged with pathogen-associated molecular patterns Rojas, MV; Camus, H; Guzman, F; Mercado L. Article ISI JOURNAL OF FISH DISEASES (2014)
USE OF ANTIGENIC PEPTIDES FOR THE DETECTION OF IMMUNOLOGICAL MARKERS IN SALMONIDS Guzman, F; Santana, P; Alvarez, C; Mercado L. Article JOURNAL OF PEPTIDE SCIENCE (2014)
Detection of the hepcidin prepropeptide and mature peptide in liver of rainbow trout Alvarez CA; Santana, PA; Guzman, F; Marshall, S; Mercado L. Article ISI SCOPUS Developmental & Comparative Immunology (2013)
Development of a sandwich ELISA for quantifying hepcidin in Rainbow trout Santana, PA; Alvarez CA; Guzman, F; Mercado L. Article ISI SCOPUS Fish & Shellfish Immunology (2013)
Development of a sandwich ELISA for quantifying hepcidin in Rainbow trout Santana, PA; Alvarez, CA; Guzman, F; Mercado, L Article FISH & SHELLFISH IMMUNOLOGY (2013)
Sb(V) and Sb(III) distribution in human erythrocytes: Speciation methodology and the influence of temperature, time and anticoagulants Quiroz W.; Aguilar L; Barria, M.; Veneciano, J; Martinez, D.; Bravo M.; Lobos, MG; Mercado L. Article ISI SCOPUS Talanta (2013)
Sb(V) and Sb(III) distribution in human erythrocytes: Speciation methodology and the influence of temperature, time and anticoagulants Quiroz, W; Aguilar, L; Barria, M; Veneciano, J; Martinez, D; Bravo, M; Lobos, MG; Mercado, L Article TALANTA (2013)
Anti-peptide antibodies: A tool for detecting IL-8 in salmonids Santana, P.; Palacios, C; Narvaez, E; Guzman, F; Gallardo J.A.; Mercado L. Article ISI ELECTRONIC JOURNAL OF BIOTECHNOLOGY (2012)
Development of a caspase-3 antibody as a tool for detecting apoptosis in cells from rainbow trout (Oncorhynchus mykiss) ROJAS, V; Guzman, F; Valenzuela C.; Marshall S.H.; Mercado L. Article ISI SCIELO SCOPUS ELECTRONIC JOURNAL OF BIOTECHNOLOGY (2012)
Development of a new antibody for detecting natural killer enhancing factor (NKEF)-like protein in infected salmonids Bethke, J; ROJAS, V; Berendsen, J; Cárdenas C; Guzman, F; Gallardo J.A.; Mercado L. Article ISI SCOPUS Journal of Fish Diseases (2012)
Development of a new antibody for detecting natural killer enhancing factor (NKEF)-like protein in infected salmonids. Bethke, J; Rojas, V; Berendsen, J; Cárdenas, C; Guzmán, F; Gallardo, J A; Mercado, L Article Journal of fish diseases (2012)
Immunological strategy for detecting the pro-inflammatory cytokine TNF-alpha in salmonids ROJAS, V; Morales-Lange, B; Guzman, F; Gallardo J.A.; Mercado L. Article ISI SCIELO SCOPUS ELECTRONIC JOURNAL OF BIOTECHNOLOGY (2012)
Parameters to evaluate the immunostimulant effect of glucan in salmonid macrophages Andres Abarca, Jorn Bethke, Roberto Flores & Luis Mercado. Article Latin American Journal of Aquatic Research (2012)
Parameters to evaluate the immunostimulant effect of Zymosan A in head kidney leucocytes (HKL) of salmonids Abarca, Andrés; Bethke, Jörn; Narváez, Edgar; Flores, Roberto; Mercado, Luis Article ISI SCIELO LATIN AMERICAN JOURNAL OF AQUATIC RESEARCH (2012)
Viral hemorrhagic septicaemia virus (VHSV) up-regulates the cytotoxic activity and the perforin/granzyme pathway in the rainbow trout RTS11 cell line Ordas, MC; Cuesta A.; Mercado, L; Bols, NC; Tafalla C. Article FISH & SHELLFISH IMMUNOLOGY (2011)
Viral hemorrhagic septicaemia virus (VHSV) up-regulates the cytotoxic activity and the perforin/granzyme pathway in the rainbow trout RTS11 cell line Ordas, MC; Cuesta, A; Mercado L.; Bols, NC; Tafalla C. Article ISI SCOPUS Fish & Shellfish Immunology (2011)
An immunological method for quantifying antibacterial activity in Salmo salar (Linnaeus, 1758) skin mucus Narvaez, E; Berendsen, J; Guzman, F; Gallardo, JA; Mercado L. Article ISI SCOPUS Fish & Shellfish Immunology (2010)
A novel antifungal peptide designed from the primary structure of a natural antimicrobial peptide purified from Argopecten purpuratus hemocytes Arenas, G.; Guzman, F; Cárdenas C; Mercado L.; Marshall, SH Article ISI SCOPUS PEPTIDES (2009)
Characterization and functional recovery of a novel antimicrobial peptide (CEC dir-CEC ret) from inclusion bodies after expression in Escherichia coli Schmitt, P.; Mercado L.; Diaz M.; Guzman, F; Arenas, G.; Marshall S.H. Article PEPTIDES (2008)
Characterization and functional recovery of a novel antimicrobial peptide (CECdir-CECret) from inclusion bodies after expression in Escherichia coli Schmitt, P.; Mercado L.; Diaz M.; Guzman, F; Arenas, G.; Marshall, SH Article ISI SCOPUS PEPTIDES (2008)
Characterization and functional recovery of a novel antimicrobial peptide (CECdir-CECret) from inclusion bodies after expression in Escherichia coli Schmitt, P; Mercado, L; Diaz, M; Guzman, F; Arenas G.; Marshall, SH Article PEPTIDES (2008)
Gill tissues of the mussel Mytilus edulis chilensis: A new source for antimicrobial peptides Mercado L.; Schmitt, P.; Marshall, SH; Arenas, G. Article SCOPUS ELECTRONIC JOURNAL OF BIOTECHNOLOGY (2005)
Phorbol esters induce translocation of the nPKC p105 to membrane in mussel hemocytes Luis Mercado; Asunción Cao; Ramiro Barcia; Juan Ignacio Martínez-Ramos Article ISI MOLECULAR AND CELLULAR BIOCHEMISTRY (2003)
Primary cultures of hemocytes from Mytilus galloprovincialis Lmk.: expression of IL-2R alpha subunit Cao, A; Mercado L.; Ramos-Martinez, JI; Barcia, R Article ISI SCOPUS Aquaculture (2003)
Detection of phenoloxidase (PO) in hemocytes of the clam Venus antiqua Mercado LA; Marshall, SH; Arenas, GM Article ISI SCOPUS MALACOLOGIA (2002)
Regulatory properties of p105: a novel PKC isoenzyme in mantle tissue from marine mussels Mercado, Luis; Cao, Asunción; Barcia, Ramiro; Ramos-Martinez, Juan Ignacio Article Biochemistry and Cell Biology (2002)
Immunological detection of a CK2 activity in Tapes semidecussatus (Mollusca, Bivalvia) mantle Mercado L.; Itarte, E; Marshall, S; Arenas, G. Article ISI SCOPUS MALACOLOGIA (2001)
[Binding of the antileukemia drug Escherichia coli L-asparaginase to the plasma membrane of normal human mononuclear cells]. Mercado-Vianco, L; Arenas-Díaz, G Article Sangre (1999)
[Escherichia coli L-asparaginase induces phosphorylation of endogenous polypeptides in human immune cells]. Mercado, L; Arenas, G Article Sangre (1999)
Binding of the antileukaemic drug L-asparaginase from escherichia coli to the plasma membrane of normal human mononuclear cells Asociación de la droga antileucémica L-asparraginasa de escherichia col a la membrana plasmática de células mononucleares humanas normales Arenas-Diaz G.; Mercado-Vianco L Article Sangre (1999)
Escherichia coli L-asparaginase induces phosphorylation of endogenous polypeptides in human immune cells L-asparaginasa de Escherichia coli induce fosforilación de polipéptidos endógenos en células inmunitarias humanas Arenas, G.; Mercado L. Article Sangre (1999)
Intracellular Ca 2+ homeostasis in rat round spermatids Berrios J.; Osses N.; Opazo C.; Reyes, J.G.; Arenas, G.; Mercado L.; Benos D.J. Article BIOLOGY OF THE CELL (1998)
Intracellular Ca2+ homeostasis in rat round spermatids. Berrios, J; Osses, N; Opazo, C; Arenas, G; Mercado, L; Benos, D J; Reyes, J G Article Biology of the cell / under the auspices of the European Cell Biology Organization (1998)
Bacterial aspartate kinase-like activity in human platelet. Arenas-Díaz, G; Mercado, L A; Marshall, S H Article Cellular & molecular biology research (1995)


A peptide derived from the D1 domain of Vibrio anguillarum flagellin modulates cytokine expression in gilthead seabream and rainbow trout Gonzalez-Stegmaier, R; Guzman, E; Romero A.; Mulero V.; Mercado L. Abstract Fish & Shellfish Immunology (2013)
Antimicrobial activity of synthetic hepcidin and hepatic response to bacterial infection in trout Alvarez C.; Guzman, F; Marshall, S; Mercado L. Abstract Fish & Shellfish Immunology (2013)
Development of an ELISA sandwich assay for quantifying IFN-gamma in salmonids Bethke, J; Barría A.; Cabrejos ME; Guzman, F; Mercado L. Abstract Fish & Shellfish Immunology (2013)
Identifying immunological markers present in early developmental stages of rainbow trout (Oncorhynchus mykiss) Santana, PA; Guzman, F; Mercado L. Abstract Fish & Shellfish Immunology (2013)
Immunostimulatory effect of beta-glucans in rainbow trout (Oncorhynchus mykiss) Schmitt, P.; Morales-Lange, B; Bethke, J; Mercado L. Abstract Fish & Shellfish Immunology (2013)
Proinflammatory caspase-1 activation in rainbow trout (Oncorhynchus mykiss) infected by fish pathogen from the genus Aeromonas ROJAS, V; Camus, H; Guzman, F; Mercado L. Abstract Fish & Shellfish Immunology (2013)
Antibacterial activity of short cationic model peptides Guzman, F; Marshall, S; Cárdenas C; Arenas, G.; Mercado L.; Albericio, F. Abstract ISI JOURNAL OF BIOTECHNOLOGY (2010)
Epitope design and chemical synthesis to develop antibodies against heat shock protein (Hsp70) in different animal models of stress Guzman, F; Berendsen, J; Gonzalez, M.; Arenas, G.; Mercado L. Abstract ISI New Biotechnology (2009)
Antibacterial activity of peptide analogs from a synthetic antifungal peptide derived from an immunogenic molecule from Agropecten purpuratus (North Chilean Scallop) Arenas, G.; Guzman, F; Mercado L.; Palacios, C; Marshall, S Abstract ISI JOURNAL OF BIOTECHNOLOGY (2007)