On the kinetics of phase separation in aqueous two-phase systems

Salamanca M.H.; Andrews B.A.; Asenjo, J.A; Merchuk J.C.

Keywords: magnesium, kinetics, water, separation, protein, salts, chloride, bacterial, phase, sodium, sulfate, article, subtilis, analytical, analysis, bacillus, mathematics, priority, journal, solution, Chemistry,, aqueous, macrogol


The effect of the tie-line location (phase volume ratio) on the kinetics of phase separation in batch PEG/salt aqueous two-phase systems (ATPS) has been investigated. PEG/sulphate systems with a stability ratio (sr) of 0.34 and 0.37 and relative tie-line lengths in the range 0.1 to 0.6 for a continuous top phase and in the range 0.03 to 0.15 for a continuous bottom phase were used in the batch studies. A continuous settler was designed with three different inlet geometries. Phase separation is much faster when the bottom phase is continuous and in this case the location on the tie-line and the presence or absence of Bacillus subtilis extract makes little difference. When the top phase is continuous the relative sizes of the phases (phase ratio, R, relative distance on tie-line, rd) has an important effect, the larger the top phase (larger R and rd) the slower the phase separation. The presence of Bacillus extract also makes the operation slower which is more marked at the largest values of R (and rd). At the largest volume ratios (R or rd) three different settling regions have been recognised, a region of coalescence, a region of drops moving to the interphase and a region where drops queue at the interphase to coalesce into the large phase. A modified correlation that takes into account the location on the tie-line and thus volume ratio (R) and relative distance (rd) has been proposed and successfully tested. The behavior of batch and continuous systems in the presence and absence of Bacillus subtilis extract in systems with continuous bottom phase was also studied. The settling velocity was lower in the continuous than in the batch systems, and in both cases the initial rate was lower in the presence of Bacillus extract.

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Título de la Revista: Journal of Chromatography B: Biomedical Applications
Volumen: 711
Número: 1-2
Editorial: Society of Laparoendoscopic Surgeons
Fecha de publicación: 1998
Página de inicio: 319
Página final: 329
URL: http://www.scopus.com/inward/record.url?eid=2-s2.0-0032569002&partnerID=q2rCbXpz