Acute activation of Maxi-K channels (hSlo) by estradiol binding to the ? subunit

Valverde M.A.; Rojas P.; Amigo, J; Cosmelli, D; Orio, P; Bahamonde M.I.; Vergara C.; Latorre R.; Mann G.E.

Keywords: muscle, transport, hormone, animals, binding, complex, culture, ion, rats, protein, cell, channel, subunits, chain, calcium, alpha, beta, laevis, channels, line, estradiol, site, humans, transduction, potassium, patch-clamp, cattle, xenopus, electrophysiology, assembly, female, signal, affinity, article, large-conductance, smooth, oocyte, techniques, vascular, animal, priority, nonhuman, journal, Animalia, RNA,, Messenger, tone, Channels,, Calcium-Activated


Maxi-K channels consist of a pore-forming ? subunit and a regulatory ? subunit, which confers the channel with a higher Ca2+ sensitivity. Estradiol bound to the ? subunit and activated the Maxi-K channel (hSlo) only when both ? and ? subunits were present. This activation was independent of the generation of intracellular signals and could be triggered by estradiol conjugated to a membrane-impenetrable carrier protein. This study documents the direct interaction of a hormone with a voltage-gated channel subunit and provides the molecular mechanism for the modulation of vascular smooth muscle Maxi-K channels by estrogens.

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Título de la Revista: SCIENCE
Volumen: 285
Número: 5435
Fecha de publicación: 1999
Página de inicio: 1929
Página final: 1931