The ependymocytes of the bovine subcommissural organ are functionally coupled through gap junctions
Keywords: system, neurons, junction, animals, culture, cell, glycoprotein, nucleus, fibers, nuclei, cattle, connexin, nerve, article, gap, ependyma, junctions, techniques, raphe, organ, controlled, animal, study, priority, nonhuman, journal, Nervous, serotoninergic, subcommissural, 43, peptidergic
The subcommissural organ (SCO) is a circunventricular organ secreting glycoproteins into the ventricle. It is richly innervated by (1) serotonergic fibers originated in raphe nuclei, that would exert an inhibitory control, and (2) peptidergic fibers of unknown function. Due to the scarce number of the latter, their functional significance might largely depends on whether the cells of the SCO are functionally coupled through gap junctions. This investigation was designed to answer this question. The bovine SCO, either freshly isolated or maintained in organ culture, was processed for immunoblot and immunocytochemistry, using an anti-connexin43 antibody, and dye coupling studies. It was found that the cells of the SCO in situ are functionally coupled through gap junctions made at least of connexin43, but in cultured explants are not. The possibility that coupling of the SCO may be controlled by the neural input and undergoes circadian variations is discussed.
|Título de la Revista:||NEUROSCIENCE LETTERS|
|Editorial:||ELSEVIER SCI IRELAND LTD|
|Fecha de publicación:||1999|
|Página de inicio:||175|