Environmental acclimatization of the carp modulates the transcription of ?-actin

Leal, S.; Quezada C.; Kausel G.; Vera, M. I.; Krauskopf, M; Sarmiento J.; Figueroa J.

Keywords: sequence, dna, animals, expression, transcription, gene, chain, beta, hybridization, parameters, variation, polymerase, carp, acclimatization, rna, article, actin, base, primers, environmental, genetic, ribosomal, reverse, physiological, seasonal, priority, Reaction, in, nonhuman, journal, RNA,, Transcription,, Messenger, Adaptation,, Carps, Situ, Transcriptase, ribosome, Cyprinus, carpio, Actins, housekeeping

Abstract

A cascade of mechanisms involving changes in gene expression are substantial to shape the adaptive responses that a eurythermal fish requires upon environmental changes in its habitat. We have previously shown that the cyclical reprogramming of rRNA transcription is a remarkable feature in carp under seasonal acclimatization. Using in situ hybridization and competitive RT-PCR we found significant differences in ?-actin transcripts, generally accepted to be coded by a typical housekeeping gene, in tissues from summer- and winter-acclimatized carp. The physiological differential ?-actin transcription herein reported places us on the alert for the reference genes estimated to be constitutive to quantitatively assess gene transcripts. © 2000 Wiley-Liss, Inc.

Más información

Título según SCOPUS: Environmental acclimatization of the carp modulates the transcription of ?-actin
Título de la Revista: JOURNAL OF CELLULAR BIOCHEMISTRY
Volumen: 80
Número: 2
Editorial: WILEY-BLACKWELL
Fecha de publicación: 2000
Página de inicio: 223
Página final: 228
Idioma: English
URL: http://www.scopus.com/inward/record.url?eid=2-s2.0-0034532539&partnerID=q2rCbXpz
DOI:

10.1002/1097-4644(20010201)80:2<223::AID-JCB110>3.0.CO;2-O

Notas: SCOPUS