Functional inhibition of native volume-sensitive outwardly rectifying anion channels in muscle cells and Xenopus oocytes by anti-CIC-3 antibody
Keywords: muscle, transport, mouse, heart, fiber, animals, expression, antibody, cells, antigen, ion, protein, cell, chloride, channel, antibodies, mechanism, physiology, mice, laevis, channels, artery, injection, strain, xenopus, oocytes, female, anions, carrier, dialysis, article, myocardium, anion, pig, dog, smooth, pulmonary, oocyte, type, controlled, injections, vascular, animal, current, study, priority, nonhuman, journal, Muscle,, Smooth,, polyclonal, Guinea, 3t3, regulatory
1. Intracellular dialysis of NIH/3T3 cells with a commercially available anti-ClC-3 polyclonal antibody (Ab) for ?30 min completely inhibited expressed guinea-pig ClC-3 currents (IgpClC-3), while intracellular dialysis with antigen-preabsorbed anti-ClC-3 Ab failed to affect IgpClC-3. 2. Anti-ClC-3 Ab was used as a selective probe to examine the relationship between endogenous ClC-3 expression and native volume-sensitive outwardly rectifying anion channels (VSOACs) in guinea-pig cardiac cells, canine pulmonary arterial smooth muscle cells (PASMCs) and Xenopus laevis oocytes. Intracellular dialysis or injection of anti-ClC-3 Ab abolished native VSOAC function in cardiac cells and PASMCs and significantly reduced VSOACs in oocytes. In contrast, native VSOAC function was unaltered by antigen-preabsorbed anti-ClC-3 Ab. 3. It is suggested that endogenous ClC-3 represents a major molecular entity responsible for native VSOACs in cardiac and smooth muscle cells and Xenopus oocytes. Anti-CIC-3 Ab should be a useful experimental tool to directly test the relationship between endogenous ClC-3 expression and native VSOAC function, and help resolve existing controversies related to the regulation and physiological role of native VSOACs in a wide variety of different cells.
|Título según SCOPUS:||Functional inhibition of native volume-sensitive outwardly rectifying anion channels in muscle cells and Xenopus oocytes by anti-CIC-3 antibody|
|Título de la Revista:||JOURNAL OF PHYSIOLOGY-LONDON|
|Fecha de publicación:||2001|
|Página de inicio:||437|