The -308 polymorphism in the promoter region of the tumor necrosis factor-alpha (TNF-?) gene and ex vivo lipopolysaccharide-induced TNF-? expression in patients with aggressive periodontitis and/or type 1 diabetes mellitus

Pérez, C.; González F.E.; Aguirre A.; Cruzat, A; Contreras-Levicoy, J; Dotte, A; Aravena, O; Salazar, L; Catalan, D; Cuenca J.; Ferreira A.; Aguillon, J.C.; Pavez, V; Araya A.V.; Schiattino I.

Keywords: insulin, polymorphism, expression, lipopolysaccharide, regions, culture, blood, gene, length, disease, mellitus, chain, single, alpha, periodontitis, association, diabetes, tumor, humans, human, male, polymerase, adult, female, restriction, genotype, necrosis, factor-alpha, article, factor, promoter, predisposition, leukocytes, fragment, genetic, type, controlled, clinical, study, 1, nucleotide, Reaction, examination, (Genetics), Lipopolysaccharides, Polymorphism,, dependent, Mellitus,, to

Abstract

Several single-nucleotide polymorphisms (SNPs) have been identified in the TNF-? gene promoter. The transition G?A at position -308 generates the TNF-?1 (G/G) and TNF-?2 (G/A or A/A) alleles, where the polymorphic TNF-?2 allele is associated with a high, in vitro TNF-? expression and an increased susceptibility to diverse illnesses. Here we study the association of the -308 TNF-? SNP with the susceptibility for developing aggressive periodontitis (AP), AP combined with type 1 diabetes mellitus (DM) and DM. We also explore the TNF-? capability expression and the presence of the -308 polymorphism. For this purpose we recruited 27 individuals with AP (AP+ group), 27 individuals with AP combined with DM (AP+/DM+ group), and 27 individuals with DM without signs of periodontitis upon clinical examination (DM+ group). The control group was comprised of 30 subjects. Genotyping for TNF-? promoter was performed by PCR-RFLP analysis. For TNF-? expression we used a blood culture system.

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Título según SCOPUS: The -308 polymorphism in the promoter region of the tumor necrosis factor-alpha (TNF-?) gene and ex vivo lipopolysaccharide-induced TNF-? expression in patients with aggressive periodontitis and/or type 1 diabetes mellitus
Título de la Revista: European Cytokine Network
Volumen: 15
Número: 4
Editorial: Libbey
Fecha de publicación: 2004
Página de inicio: 364
Página final: 370
Idioma: English
URL: http://www.scopus.com/inward/record.url?eid=2-s2.0-19944433491&partnerID=q2rCbXpz
Notas: SCOPUS