Marlin-1 is expressed in testis and associates to the cytoskeleton and GABA B receptors

Vidal R.L.; Ramirez A.; Castro M.; Concha I.I.; couve, a

Keywords: acid, proteins, rat, mouse, distribution, animals, expression, transcription, binding, cells, rats, protein, cell, microtubule, chain, spermatogenesis, testis, spermatozoa, mice, immunoprecipitation, rodentia, immunohistochemistry, male, receptor, polymerase, interaction, rna-binding, tissue, tubule, cytoskeleton, microtubules, sertoli, drug, article, kinase, spermatozoon, cellular, animal, reverse, c, 4, 1, priority, Reaction, nonhuman, journal, Receptors,, Rats,, Sprague-Dawley, aminobutyric, Inbred, Mice,, unclassified, Seminiferous, Microtubule-Associated, BALB, b, Janus, GABA-B, Marlin


Marlin-1 is a GABA B receptor and Jak tyrosine kinase-binding protein that also associates with RNA and microtubules. In humans and rodents, expression of Marlin-1 is predominantly restricted to the brain, but expression in lymphoid cells has also been reported. Here, we have studied the distribution of Marlin-1 in testis and spermatozoa. Our results indicate that Marlin-1 is highly expressed in testis. The protein is abundant in spermatogonia, spermatocytes, spermatozoa, and Sertoli cells. We also have studied the subcellular distribution in spermatozoa. Marlin-1 is present in the tail and to a lesser degree in the head of the sperm cell. Finally, we have explored two protein interactions. Our findings demonstrate that Marlin-1 associates with a microtubule fraction and with GABA B receptors in testis suggesting that the set of protein interactions of Marlin-1 are conserved in different tissues. © 2007 Wiley-Liss, Inc.

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Volumen: 103
Número: 3
Editorial: Wiley
Fecha de publicación: 2008
Página de inicio: 886
Página final: 895