The availability of surface GABAB receptors is independent of ?-aminobutyric acid but controlled by glutamate in central neurons

Vargas K.J.; Tello J.A.; couve, a; Terunuma, M; Moss S.J.; Pangalos, M. N.

Keywords: acid, neurons, rat, membrane, fiber, endocytosis, animals, transmission, degradation, complex, plasma, brain, rats, protein, cell, pathways, pregnancy, acids, surfaces, physiology, metabolism, surface, male, receptor, sugar, membranes, cytology, bodies, nerve, hippocampus, female, proteasome, recycling, cortex, dendrite, synapse, plasmas, article, dimers, synapses, hippocampal, clathrin, controlled, dynamin, animal, study, 4, amino, priority, nonhuman, journal, gamma-Aminobutyric, Receptors,, Rats,, Sprague-Dawley, Sprague, Dawley, aminobutyric, and, I, Cerebral, glutamic, b, Synaptic, (sucrose), Endopeptidase, cortical, transmissions, GABA-B, Hetero, Neuro-transmitters


The efficacy of synaptic transmission depends on the availability of ionotropic and metabotropic neurotransmitter receptors at the plasma membrane, but the contribution of the endocytic and recycling pathways in the regulation of ?-aminobutyric acid type B (GABAB) receptors remains controversial. To understand the mechanisms that regulate the abundance of GABAB receptors, we have studied their turnover combining surface biotin labeling and a microscopic immunoendocytosis assay in hippocampal and cortical neurons. We report that internalization of GABAB receptors is agonist-independent. We also demonstrate that receptors endocytose in the cell body and dendrites but not in axons. Additionally, we show that GABA B receptors endocytose as heterodimers via clathrin- and dynamin-1-dependent mechanisms and that they recycle to the plasma membrane after endocytosis. More importantly, we show that glutamate decreases the levels of cell surface receptors in a manner dependent on an intact proteasome pathway. These observations indicate that glutamate and not GABA controls the abundance of surface GABAB receptors in central neurons, consistent with their enrichment at glutamatergic synapses. © 2008 by The American Society for Biochemistry and Molecular Biology, Inc.

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Volumen: 283
Número: 36
Editorial: Elsevier
Fecha de publicación: 2008
Página de inicio: 24641
Página final: 24648