First results in the development of a genetic marker based on the storage proteins in two species of the Nothofagus genus Primeros resultados en el desarrollo de un marcador genético basado en las proteínas de reserva en dos especies del género Nothofagus

Martin M.A.; Munoz S.; Martín L.M.; Alvarez J.B.; Molina J.R.; Herrera M.Á.; Muñoz F; Uribe M.

Keywords: nothofagus, glauca, alessandri, Nothofagaceae

Abstract

The Nothofagus genus (Nothofagaceae) in Chile includes 12 species and some hybrids among them. Some of these species are seriously threatened, presenting a low distribution and high genetic erosion. In this study a molecular marker based on seed storage proteins Nothofagus sp. was developed. Seed samples of Nothofagus alessandrii and N. glauca from two locations in southern Chile were analysed for each species. Among the different fractions of seed proteins (albumins, globulins, prolamins and glutelins) analyzed, the glutelins showed the best results, presenting conspicuous bands. Up to 39 bands were detected in both species; 22 in N. alessandrii (including 14 polymorphic) and 26 in N. glauca (21 polymorphic), with seven of them common to both species. There was a clear difference between the protein profiles of both species; we detected a preliminary value of genetic diversity in N. glauca greater than in N. alessandrii. These results, although preliminary, suggest that this marker could be useful for assessing genetic diversity in the Nothofagus genus.

Más información

Título de la Revista: BOSQUE
Volumen: 31
Número: 3
Editorial: Facultad de Ciencias Forestales y Recursos Naturales de la Universidad Austral de Chile.
Fecha de publicación: 2010
Página de inicio: 252
Página final: 257
URL: http://www.scopus.com/inward/record.url?eid=2-s2.0-79951783242&partnerID=q2rCbXpz