Linoleic acid peroxidation initiated by Fe 3+-reducing compounds recovered from Eucalyptus grandis biotreated with Ceriporiopsis subvermispora

Horta M.A.; Masarin F.; Ferraz A.; Rodriguez J.

Keywords: iron, oxidation, acid, system, solvent, size, chromatography, in-vitro, lipids, degradation, solvents, ion, tree, absorption, disease, acids, peroxidation, biodegradation, mass, ultraviolet, radiation, wood, extraction, solubilization, fungi, fragments, fungal, extracts, lignin, column, molecular, data, apparent, microbiology, analysis, eucalyptus, lipid, activity, evergreen, fungus, grandis, lignins, exclusion, Reaction, fatty, reactions, Ceriporiopsis, subvermispora, aqueous, linoleic, molar, Reducing, Coriolaceae, Bio-pulping, Ball-milled


This work evaluates linoleic acid peroxidation reactions initiated by Fe 3+-reducing compounds recovered from Eucalyptus grandis, biotreated with the biopulping fungus Ceriporiopsis subvermispora. The aqueous extracts from biotreated wood had the ability to reduce Fe 3+ ions from freshly prepared solutions. The compounds responsible for the Fe 3+-reducing activity corresponded to UV-absorbing substances with apparent molar masses from 3kDa to 5kDa. Linoleic acid peroxidation reactions conducted in the presence of Fe 3+ ions and the Fe 3+-reducing compounds showed that the rate of O 2 consumption during peroxidation was proportional to the Fe 3+-reducing activity present in each extract obtained from biotreated wood. This peroxidation reaction was coupled with in-vitro treatment of ball-milled E. grandis wood. Ultraviolet data showed that the reaction system released lignin fragments from the milled wood. Size exclusion chromatography data indicated that the solubilized material contained a minor fraction representing high-molar-mass molecules excluded by the column and a main low-molar-mass peak. Overall evaluation of the data suggested that the Fe 3+-reducing compounds formed during wood biodegradation by C. subvermispora can mediate lignin degradation through linoleic acid peroxidation. © 2010 Elsevier Ltd.

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Volumen: 65
Número: 1
Editorial: Society of Laparoendoscopic Surgeons
Fecha de publicación: 2011
Página de inicio: 164
Página final: 171