Glucose effect in the expression of endothelial lipase in human endothelial cells and in patients with diabetes mellitus type 2 Efecto de glucosa en la expresión de lipasa endotelial en células endotelialeshumanas y en sujetos con diabetes mellitus tipo 2

Pierart, C; Serrano, V; Rubio, L.; Foncea, R; Ebensperger, R

Keywords: enzymology, lipase, insulin, endothelium, biosynthesis, glucose, cells, culture, hemoglobin, blood, cell, mellitus, metabolism, diabetes, humans, human, male, level, aged, adult, female, drug, article, endothelial, vein, umbilical, triacylglycerol, type, middle, 2, effect, Cells,, Cultured, and, Aged,, 80, over, non, dependent, Mellitus,, glycosylated, A,


Introduction: Endothelial Lipase (EL), enzyme thatmodulates HDL metabolism, is overregulated by inflammatory-cytokines. Type 2 Diabetes (DM2) has been associatedwith a subclinical inflammation, so it has beenruled that these patients could have high levels of EL. Theobjectives of the research are to determine the effect ofglucose in the expression of EL in culturing cells and evaluatethe relation between the levels of EL and the metaboliccontrol in patients with DM2.Method: During 24 hours, human endothelial cells(HUVEC) were stimulated with different concentrationsof glucose (5.5, 25 and 50 mmol/L), the effect was evaluatedover the expression of EL. In DM2 patients levels ofEL, glucose and HbA1c were measured. We had a controlgroup (8) to determine the levels of enzyme. EL was measuredby immune transference, and the results wereexpressed by arbitrary units(AU).Results: In HUVEC cells, the expression of EL wasdirectly proportional extracellular glucose (p < 0.05). 24diabetic patients were evaluated (15 females and 9 males)average age from 60 ± 9,7 years old. The studied groupshowed levels of EL bigger than the control group(14911AU and 10250, 18AU) respectively (p < 0.05). Wefound no relation between glucose, HbA1c and EL.Conclusion: In HUVEC cells there is a direct relationbetween extracell glucose and EL. The diabetic patientshad higher levels of EL than the control group, but thesewas not related with glucose or HbA1c, these shows theexistence of other factors that participate in the increasementof EL.

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Volumen: 26
Número: 4
Editorial: Aran Ediciones SA
Fecha de publicación: 2011
Página de inicio: 916
Página final: 921