2-Aminoethoxydiphenyl borate (2-APB) reduces alkaline phosphatase release, CD63 expression, F-actin polymerization and chemotaxis without affecting the phagocytosis activity in bovine neutrophils

Conejeros I.; Velasquez Z.D.; Carretta M.D.; Alarcón P.; Hidalgo M. A.; burgos R.A.

Abstract

2-Aminoethoxydiphenyl borate (2-APB) interferes with the Ca 2+ influx and reduces the ROS production, gelatinase secretion and CD11b expression in bovine neutrophils. Moreover, it has been suggested that inhibition of the Ca 2+ channel involved in the store operated Ca 2+ entry (SOCE) is a potential target for the development of new anti-inflammatory drugs in cattle, however it is unknown whether 2-APB affects neutrophil functions associated with the innate immune response. This study describes the effect of 2-APB, a putative SOCE inhibitor, on alkaline phosphatase activity a marker of secretory vesicles, CD63 a marker for azurophil granules, F-actin polymerization and in vitro chemotaxis in bovine neutrophils stimulated with platelet-activating factor (PAF). Also, we evaluated the effect of 2-APB in the phagocytic activity against Escherichia coli and Staphylococcus aureus bioparticles. We observed that doses of 2-APB ≥10μM significantly reduced alkaline phosphatase activity and in vitro chemotaxis, whereas concentrations of 2-APB ≥50μM reduced CD63 expression and F-actin polymerization. Finally, we observed that 2-APB did not affect the phagocytic activity in neutrophils incubated with E. coli and S. aureus bioparticles. We concluded that inhibition of Ca 2+ influx could be a useful strategy to reduce inflammatory process in cattle. © 2012 Elsevier B.V.

Más información

Título según WOS: 2-Aminoethoxydiphenyl borate (2-APB) reduces alkaline phosphatase release, CD63 expression, F-actin polymerization and chemotaxis without affecting the phagocytosis activity in bovine neutrophils
Título según SCOPUS: 2-Aminoethoxydiphenyl borate (2-APB) reduces alkaline phosphatase release, CD63 expression, F-actin polymerization and chemotaxis without affecting the phagocytosis activity in bovine neutrophils
Título de la Revista: VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
Volumen: 145
Número: 01-feb
Editorial: ELSEVIER SCIENCE BV
Fecha de publicación: 2012
Página de inicio: 540
Página final: 545
Idioma: English
URL: http://www.scopus.com/inward/record.url?eid=2-s2.0-84856546968&partnerID=40&md5=90249ab46c06eb5fd472116b2498cb88
DOI:

10.1016/j.vetimm.2011.12.006

Notas: ISI, SCOPUS