Detección y tipificación de virus papiloma humano en lesiones preneoplásicas de cuello uterino

LÓPEZ M, JAIME; Ili G, Carmen Gloria; Brebi M, Priscilla; GARCÍA M, PATRICIA; CAPURRO V, ITALO; GUZMÁN G, PABLO; SUÁREZ P, EUGENIO; OJEDA F, JOSÉ MANUEL; Roa S, Juan Carlos

Abstract

The relationship between human papillomavirus (HPV) and uterine cervical cancer (UCC) is widely known and accepted. Aim: To determine the frequency of genotypes of HPV in cervical preneoplastic lesions in a high risk area of UCC. Material and Methods: Using a combination of PCR and Reverse Line Blot technique, 235 formalin fixed paraffin embedded samples, with diagnosis of low-grade squamous intraepithelial lesion (LSIL) or high-grade squamous intraepithelial lesion (HSIL) were genotyped. Results: HPV was detected in 61.2% of LSIL and 78.1% of HSIL. The main genotypes found were HPV 16, 18, 31, 45, 56 y 58. HPV 16 was the most common in both LSIL (18.1%) and HSIL (36.9%). HPV 16 or 18 were present in 25.1% and 47.1% of the LSIL and HSIL respectively. In both LSIL and HSIL, the predominant viral genotypes were those types classified as with a high oncogenic risk. Conclusions: HPV genotypes 16, 18, 31, 45, 56 y 58 were the most common in our series. HPV 16 and 18, viral types with high oncogenic risk and included in commercial vaccines, were found in 25.1% and 47.1% of LSIL and HSIL, respectively.

Más información

Título según SCIELO: Detección y tipificación de virus papiloma humano en lesiones preneoplásicas de cuello uterino
Título de la Revista: Revista médica de Chile
Volumen: 138
Número: 11
Editorial: Sociedad Médica de Santiago
Fecha de publicación: 2010
Página de inicio: 1343
Página final: 1350
Idioma: es
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872010001200001&lng=en&nrm=iso&tlng=en
DOI:

10.4067/S0034-98872010001200001

Notas: SCIELO