RNA interference of Marlin-1/Jakmip1 results in abnormal morphogenesis and migration of cortical pyramidal neurons

Vidal R.L.; Fuentes P.; Valenzuela, J.I.; Alvarado-Diaz C.P.; Ramirez O.A.; Kukuljan M.; couve, a

Abstract

The formation of the nervous systems requires processes that coordinate proliferation, differentiation and migration of neuronal cells, which extend axons, generate dendritic branching and establish synaptic connections during development. The structural organization and dynamic remodeling of the cytoskeleton and its association to the secretory pathway are critical determinants of cell morphogenesis and migration. Marlin-1 (Jakmip1) is a microtubule-associated protein predominantly expressed in neurons and lymphoid cells. Marlin-1 participates in polarized secretion in lymphocytes, but its functional association with the neuronal cytoskeleton and its contribution to brain development have not been explored. Combining in vitro and in vivo approaches we show that Marlin-1 contributes to the establishment of neuronal morphology. Marlin-1 associates to the cytoskeleton in neurites, is required for the maintenance of an intact Golgi apparatus and its depletion produces the down-regulation of kinesin-1, a plus-end directed molecular motor with a central function in morphogenesis and migration. RNA interference of Marlin-1 in vivo results in abnormal migration of newborn pyramidal neurons during the formation of the cortex. Our results support the involvement of Marlin-1 in the acquisition of the complex architecture and migration of pyramidal neurons, two fundamental processes for the laminar layering of the cortex. © 2012 Elsevier Inc.

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Título según WOS: RNA interference of Marlin-1/Jakmip1 results in abnormal morphogenesis and migration of cortical pyramidal neurons
Título según SCOPUS: RNA interference of Marlin-1/Jakmip1 results in abnormal morphogenesis and migration of cortical pyramidal neurons
Título de la Revista: MOLECULAR AND CELLULAR NEUROSCIENCE
Volumen: 51
Número: 01-feb
Editorial: ACADEMIC PRESS INC ELSEVIER SCIENCE
Fecha de publicación: 2012
Página de inicio: 1
Página final: 11
Idioma: English
URL: http://www.scopus.com/inward/record.url?eid=2-s2.0-84864530835&partnerID=40&md5=6ee0ce7a585b4cb9901141b05a79600c
DOI:

10.1016/j.mcn.2012.07.007

Notas: ISI, SCOPUS