Calcium and mitochondrial metabolism in ceramide-induced cardiomyocyte death

Parra, V; Moraga, F; Kuzmicic J.; López-Crisosto, C; Troncoso R.; Torrealba, N; Criollo, A; Diaz-Elizondo, J; Rothermel BA; Quest, AFG; Lavandero S.

Abstract

Ceramides are important intermediates in the biosynthesis and degradation of sphingolipids that regulate numerous cellular processes, including cell cycle progression, cell growth, differentiation and death. In cardiomyocytes, ceramides induce apoptosis by decreasing mitochondrial membrane potential and promoting cytochrome-c release. Ca2+ overload is a common feature of all types of cell death. The aim of this study was to determine the effect of ceramides on cytoplasmic Ca2+ levels, mitochondrial function and cardiomyocyte death. Our data show that C-2-ceramide induces apoptosis and necrosis in cultured cardiomyocytes by a mechanism involving increased Ca2+ influx, mitochondrial network fragmentation and loss of the mitochondrial Ca2+ buffer capacity. These biochemical events increase cytosolic Ca2+ levels and trigger cardiomyocyte death via the activation of calpains. (C) 2013 Elsevier B.V. All rights reserved.

Más información

Título según WOS: Calcium and mitochondrial metabolism in ceramide-induced cardiomyocyte death
Título según SCOPUS: Calcium and mitochondrial metabolism in ceramide-induced cardiomyocyte death
Título de la Revista: Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease
Volumen: 1832
Número: 8
Editorial: Elsevier
Fecha de publicación: 2013
Página de inicio: 1334
Página final: 1344
Idioma: English
URL: http://linkinghub.elsevier.com/retrieve/pii/S0925443913001336
DOI:

10.1016/j.bbadis.2013.04.009

Notas: ISI, SCOPUS