Antioxidant activity of the mangiferin inclusion complex with beta-cyclodextrin

Ferreira, FD; Valentim, IB; Ramones, ELC; Trevisan, MTS; Olea Azar C.; Perez-Cruz, F; de Abreu, FC; Goulart, MOF

Abstract

The aim of this study was to characterize the complex between mangiferin (MGN) with beta-cyclodextrin (beta-CD) obtained through the co-evaporation method using Differential Scanning Calorimetry (DSC) and Fourier Transform Infrared spectroscopy (FT-IR), as well as to verify the antioxidant activity of this complex by means of radical scavenging activity toward 2,2'-diphenyl-1-picrylhydrazyl radical (RSA-DPPH.), with additional analysis of solvent effects, and Oxygen Radical Antioxidant Capacity-Fluorescein (ORAC-FL) assays. The protective effects of MGN and the MGN:beta-CD complex against peroxyl radical-initiated membrane lipid peroxidation were also evaluated. The analysis of the solvent effects in the DPPH. method demonstrated that at amounts of organic solvents lower than the ratio 50:50 (methanol-water or ethanol-water) there is a drawback, due to the hydrophobic nature of DPPH and should not be used. The antioxidant activity of the MGN:beta-CD complex was higher than in its free form, as seen by both methods. The RSA-DPPH. value was twofold larger for the complex while the ORAC-FL value was fifteenfold larger. Lipid peroxidation assays showed that the MGN:beta-CD complex has a protective effect on the membrane that is as effective as the positive control (Trolox). (C) 2012 Elsevier Ltd. All rights reserved.

Más información

Título según WOS: Antioxidant activity of the mangiferin inclusion complex with beta-cyclodextrin
Título según SCOPUS: Antioxidant activity of the mangiferin inclusion complex with ?-cyclodextrin
Título de la Revista: LWT-FOOD SCIENCE AND TECHNOLOGY
Volumen: 51
Número: 1
Editorial: ELSEVIER SCIENCE BV
Fecha de publicación: 2013
Página de inicio: 129
Página final: 134
Idioma: English
URL: http://linkinghub.elsevier.com/retrieve/pii/S0023643812004124
DOI:

10.1016/j.lwt.2012.09.032

Notas: ISI, SCOPUS