Rapid Enzyme-Linked Immunosorbent Assay for the Detection of Hantavirus-Specific Antibodies in Divergent Small Mammals

Cautivo K, Schountz T, Acuña-Retamar M, Ferrés M, Torres-Pérez F.

Abstract

We assessed the utility of an enzyme-linked immunosorbent assay (ELISA) for the detection of hantavirus-specific antibodies from sera of Oligoryzomys longicaudatus, the principal reservoir of Andes virus (ANDV), using an antigen previously developed for detection of antibodies to Sin Nombre virus (SNV) in sera from Peromyscus maniculatus. The assay uses a protein A/G horseradish peroxidase conjugate and can be performed in as little as 1.5 hours. Serum samples from Oligoryzomys longicaudatus collected in central-south Chile were used and the assay identified several that were antibody positive. This assay can be used for the rapid detection of antibodies to divergent hantaviruses from geographically and phylogenetically distant rodent species.

Más información

Título según WOS: Rapid Enzyme-Linked Immunosorbent Assay for the Detection of Hantavirus-Specific Antibodies in Divergent Small Mammals
Título según SCOPUS: Rapid Enzyme-linked immunosorbent assay for the detection of hantavirus-specific antibodies in divergent small mammals
Título de la Revista: Viruses
Volumen: 6
Número: 5
Editorial: MDPI
Fecha de publicación: 2014
Página de inicio: 2028
Página final: 2037
Idioma: English
URL: http://www.mdpi.com/1999-4915/6/5/2028/
DOI:

10.3390/v6052028

Notas: ISI, SCOPUS - ISI