Glutathione reductase-mediated synthesis of tellurium-containing nanostructures exhibiting antibacterial properties.

Pugin, Benoit; Cornejo, Fabián A.; Munoz-Diaz, P; Munoz-Villagran, CM; Vargas-Perez, JI; Arenas, Felipe, A.; Vásquez, Claudio C.


Tellurium, a metalloid belonging to the group 16 of the Periodic Table, displays very interesting physical and chemical properties, and lately has attracted significant attention for its use in nanotechnology. In this context, the use of microorganisms for synthesizing nanostructures emerges as an eco-friendly and exciting approach as compared to their chemical synthesis. To generate Te-containing nanostructures, bacteria reduce enzymatically tellurite to elemental tellurium. In this work, using a classic biochemical approach, we looked for a novel tellurite reductase from the Antarctic bacterium Pseudomonas sp. BNF22 and used it to generate tellurium-containing nanostructures. A new tellurite reductase was identified as glutathione reductase, which was subsequently overproduced in E. coli. The characterization of this enzyme showed that it is a NADPH-dependent tellurite reductase, with optimum reducing activity at 30 °C and pH 9.0. Finally, the enzyme was able to generate Te-containing nanostructures, about 68 nm in size that exhibits interesting antibacterial properties against E. coli, with no apparent cytotoxicity against eukaryotic cells.

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Título de la Revista: Applied Environmental Microbiology
Volumen: 80
Número: 20
Fecha de publicación: 2014
Idioma: English
Financiamiento/Sponsor: American Society for Microbiology.
Notas: doi: 10.1128/AEM.02207-14