Sera of Chagasic patients react with antigens from the tomato parasite Phytomonas serpens

Graça-de Souza, VK; Monteiro-Góes, V; Manque P.; Souza, TA; Corrêa, PR; Buck, GA; Avila, AR; Yamauchi, LM; Pinge-Filho, P; Goldenberg, S; Krieger, MA; Yamada-Ogatta, SF


The genus Phytomonas comprises trypanosomatids that can parasitize a broad range of plant species. These fagellates can cause diseases in some plant families with a wide geographic distribution, which can result in great economic losses. We have demonstrated previously that Phytomonas serpens 15T, a tomato trypanosomatid, shares antigens with Trypanosoma cruzi, the agent of human Chagas disease. Herein, two-dimensional gel electrophoresis (2-DE) and mass spectrometry (MS) were used to identify proteins of P. serpens 15T that are recognized by sera from patients with Chagas disease. After 2D-electrophoresis of whole-cell lysates, 31 peptides were selected and analyzed by tandem mass spectrometry. Twenty-eight polypeptides were identifed, resulting in 22 different putative proteins. The identifed proteins were classifed into 8 groups according to biological process, most of which were clustered into a cellular metabolic process category. These results generated a collection of proteins that can provide a starting point to obtain insights into antigenic cross reactivity among trypanosomatids and to explore P. serpens antigens as candidates for vaccine and immunologic diagnosis studies.

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Título según SCIELO: Sera of Chagasic patients react with antigens from the tomato parasite Phytomonas serpens
Título de la Revista: BIOLOGICAL RESEARCH
Volumen: 43
Número: 2
Fecha de publicación: 2010
Página de inicio: 233
Página final: 241
Idioma: en