Development of microsatellites for Southern Darwin's frog Rhinoderma darwinii (Dumeril & Bibron, 1841)

Fuentes-Navarrete, Marina; Gomez-Uchida, Daniel; Gallardo-Escárate, Cristian; Canales-Aguirre, Cristian B.; Galleguillos, Ricardo; Ortiz, Juan Carlos

Abstract

The Southern Darwin's frog Rhinoderma darwinii is a charismatic, mouth-brooding amphibian endemic to temperate forests of South America with a Vulnerable conservation status according to the IUCN Red List. We developed microsatellite markers from next generation sequence data that will aid genetic monitoring during and after re-introduction efforts. Using bioinformatics we characterized 3,521 perfect microsatellite repeats and designed primers for 35 of them. From these, 23 were polymorphic and amplified reliably. Number of alleles varied between 2 and 15, allele sizes varied between 84 and 299 bp, and observed heterozygosities varied between 0.105 and 0.904. These microsatellites represent a valuable resource to aid recovery of threatened Southern Darwin's frog populations.

Más información

Título según WOS: Development of microsatellites for Southern Darwin's frog Rhinoderma darwinii (Dumeril & Bibron, 1841)
Título según SCOPUS: Development of microsatellites for Southern Darwin’s frog Rhinoderma darwinii (Duméril & Bibron, 1841)
Título de la Revista: CONSERVATION GENETICS RESOURCES
Volumen: 6
Número: 4
Editorial: Springer
Fecha de publicación: 2014
Página de inicio: 1035
Página final: 1037
Idioma: English
URL: http://link.springer.com/10.1007/s12686-014-0279-4
DOI:

10.1007/s12686-014-0279-4

Notas: ISI, SCOPUS