A Novel Role of Eruca sativa Mill. (Rocket) Extract: Antiplatelet (NF-kappa B Inhibition) and Antithrombotic Activities
Keywords: Antiplatelet; Antithrombotic; Eruca sativa Mill; Functional food; Platelet inflammatory mediators; Rocket
Background: Epidemiological studies have shown the prevention of cardiovascular diseases through the regular consumption of vegetables. Eruca sativa Mill., commonly known as rocket, is a leafy vegetable that has anti-inflammatory activity. However, its antiplatelet and antithrombotic activities have not been described. Methods: Eruca sativa Mill. aqueous extract (0.1 to 1 mg/mL), was evaluated on human platelets: (i) P-selectin expression by flow cytometry; (ii) platelet aggregation induced by ADP, collagen and arachidonic acid; (iii) IL-1 beta, TGF-beta 1, CCL5 and thromboxane B2 release; and (iv) activation of NF-kappa B and PKA by western blot. Furthermore, (v) antithrombotic activity (200 mg/kg) and (vi) bleeding time in murine models were evaluated. Results: Eruca sativa Mill. aqueous extract (0.1 to 1 mg/mL) inhibited P-selectin expression and platelet aggregation induced by ADP. The release of platelet inflammatory mediators (IL-1 beta, TGF-beta 1, CCL5 and thromboxane B2) induced by ADP was inhibited by Eruca sativa Mill. aqueous extract. Furthermore, Eruca sativa Mill. aqueous extract inhibited NF-kappa B activation. Finally, in murine models, Eruca sativa Mill. aqueous extract showed significant antithrombotic activity and a slight effect on bleeding time. Conclusion: Eruca sativa Mill. presents antiplatelet and antithrombotic activity.
|Título según WOS:||A Novel Role of Eruca sativa Mill. (Rocket) Extract: Antiplatelet (NF-kappa B Inhibition) and Antithrombotic Activities|
|Título según SCOPUS:||A Novel Role of Eruca sativa Mill. (rocket) extract: Antiplatelet (NF-?B Inhibition) and antithrombotic activities|
|Título de la Revista:||Nutrients|
|Fecha de publicación:||2014|
|Página de inicio:||5839|