Phenomenological modelling of hydrophobic interaction chromatography Modelación fenomenológica de cromatografía de interacción hidrofóbica

Mahn A.V.

Keywords: Chromatography Differential separation Hydrophobic interaction Protein purification

Abstract

The aim of this work was to obtain a phenomenological model to describe the elution profile in hydrophobic interaction chromatography of a protein injected as a pulse. The plate model, which was solved numerically, was used, and good fit to experimental data was found. The plate model allowed simulating the chromatographic process under different operating conditions, such as flow rate, elution gradient and sample volume. In is concluded that it is possible to apply the plate theory to describe elution profiles of proteins in hydrophobic interaction chromatography. The proposed model could be used to optimize the separation of protein mixtures.

Más información

Título según SCOPUS: Phenomenological modelling of hydrophobic interaction chromatography [Modelación fenomenológica de cromatografía de interacción hidrofóbica]
Título de la Revista: INFORMACION TECNOLOGICA
Volumen: 20
Número: 3
Editorial: EDITORIAL DEL NORTEL TDA - BRASIL 43 · 1 - LA SERENA
Fecha de publicación: 2009
Página de inicio: 135
Página final: 144
Idioma: eng
DOI:

10.1612/inf.tecnol.4081it.08

Notas: SCOPUS