The role of two superoxide dismutase mRNAs in rye aluminium tolerance

Sánchez-Parra, B.; Figueiras, A. M.; Abd El-Moneim, D.; Contreras R.; Rouco, R.; Gallego, F. J.; Benito C.; Martinoia, E.


Aluminium (Al) is the main factor that limits crop production in acidic soils. There is evidence that antioxidant enzymes such as superoxide dismutase (SOD) play a key role against Al-induced oxidative stress in several plant species. Rye is one of the most Al-tolerant cereals and exudes both citrate and malate from the roots in response to Al. The role of SOD against Al-induced oxidative stress has not been studied in rye. Al accumulation, lipid peroxidation, H2O2 production and cell death were significantly higher in sensitive than in tolerant rye cultivars. Also, we characterised two genes for rye SOD: ScCu/ZnSOD and ScMnSOD. These genes were located on the chromosome arms of 2RS and 3RL, respectively, and their corresponding hypothetical proteins were putatively classified as cytosolic and mitochondrial, respectively. The phylogenetic relationships indicate that the two rye genes are orthologous to the corresponding genes of other Poaceae species. In addition, we studied Al-induced changes in the expression profiles of mRNAs from ScCu/ZnSOD and ScMnSOD in the roots and leaves of tolerant Petkus and sensitive Riodeva rye. These genes are mainly expressed in roots in both ryes, their repression being induced by Al. The tolerant cultivar has more of both mRNAs than the sensitive line, indicating that they are probably involved in Al tolerance. © 2014 German Botanical Society and The Royal Botanical Society of the Netherlands.

Más información

Título según WOS: ID WOS:000353057300013 Not found in local WOS DB
Título según SCOPUS: ID 2-s2.0-84927698907 Not found in local SCOPUS DB
Título de la Revista: PLANT BIOLOGY
Volumen: 17
Editorial: Wiley
Fecha de publicación: 2015
Página de inicio: 694
Página final: 702