Prokaryotic Metatranscriptomics

Pérez-Pantoja, Danilo; Tamames, Javier; McGenity, Terry J; Timmis, Kenneth N; Nogales, Balbina


Metatranscriptomics, the determination of RNA transcripts found in a microbiome, is a very active field nowadays. Improvements both in sequencing technologies and in software for analysis have made metatranscriptomics a very amenable technique, soon to be routinely used. In this chapter, we illustrate the steps needed to perform a metatranscriptomic experiment, from the collection of the samples to the production of the final results. The full protocol is composed of wet-lab procedures for preparing and sequencing the samples, and in silico, bioinformatic techniques to process and analyze the results.

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Fecha de publicación: 2015
Página de inicio: 69
Página final: 98
Idioma: English


Notas: DOI: 10.1007/8623_2015_146