Prolactin gene expression and changes of prolactin pituitary level during the seasonal acclimatization of the carp.

Figueroa J.; Molina A.; Alvarez M.; Villanueva J.; Reyes A.; León G; Krauskopf, M


The effect of seasonal acclimatization on the extent of prolactin (PRL) gene expression and on the content of this was studied in summer- and winter-carp (Cyprinus carpio) hormone pituitary glands. PRL content in the rostral pars distalis (RPD) was evaluated by immunocytochemistry using antibodies against a cross-linked synthetic peptide comprising the sequence of 15 amino acids which conform to the primary structure of carp PRL. To assess the level of PRL gene transcription, a 24-mer synthetic oligonucleotide probe whose sequence included nucleotides 2041-2064 located in exon V of the carp PRL gene, was used. Employing in situ hybridization assays, a high expression of PRL mRNA was observed in the RPD of summer-acclimatized carp. A negligible level of transcription was observed in tissue sections of pituitary glands from winter-acclimatized carp. Concurrently, immunodetection of the PRL-producing cells in the RPD revealed that the pituitary hormone level was significantly higher in the warm season-adapted carp

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Título de la Revista: Comparative Biochemistry and Physiology - Part B: Biochemistry & Molecular Biology
Volumen: 108
Número: 4
Fecha de publicación: 1994
Página de inicio: 551
Página final: 560
Idioma: English
Notas: ISI