Expression of the chicken lysozyme gene in potato enhances resistance to infection by Erwinia carotovora subsp, Atroseptica

Serrano, C.; Arce Johnson P.; Torres H.; Gebauer M.; Gutiérrez M; Moreno, M.; Jordana, X; Venegas A.; Kalazich J.; Holuigue, L

Abstract

Infection of potato plants and tubers with the bacterium Erwinia carotovora subsp, atroseptica produces blackleg and soft rot diseases, which cause significant losses to crops and stored potatoes. In order to obtain resistance against this bacterium, the gene chly encoding the enzyme lysozyme from chicken was introduced into potato plants (cv. Desiree) via Agrobacterium-mediated transformation. Sixty-three and 69 transgenic potato clones were evaluated in the greenhouse for resistance to blackleg and soft rot diseases, respectively. Results reported in this paper indicate that 21%-29% of the potato clones showed increased resistance to infection by the bacterium E. c, subsp, atroseptica T7, as revealed by a reduced severity of blackleg or soft rot symptoms. Nine clones showing different levels of resistance were selected for further molecular analysis. The number of copies of the transgene integrated in the plant genome of these clones was estimated by Southern blot analysis. The level of transgene expression, detected by Northern blot analysis, correlated with the level of resistance detected in these clones.

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Título según WOS: Expression of the chicken lysozyme gene in potato enhances resistance to infection by Erwinia carotovora subsp, Atroseptica
Título según SCOPUS: Expression of the chicken lysozyme gene in potato enhances resistance to infection by Erwinia carotovora subsp. atroseptica
Título de la Revista: AMERICAN JOURNAL OF POTATO RESEARCH
Volumen: 77
Número: 3
Editorial: Springer
Fecha de publicación: 2000
Página de inicio: 191
Página final: 199
Idioma: English
Notas: ISI, SCOPUS