Chromogenic plate assay distinguishing bacteriolytic from bacteriostatic activity of an antibiotic agent

Mardones, G; Venegas A.

Abstract

A solid agar plate assay was devised to discriminate bacteriolytic from bacteriostatic activity for a given antibacterial agent. The assay uses a bacterial culture harboring beta-galactosidase enzyme as reporter of cellular lysis. When a drop of bacteriolytic compound is placed on the agar, beta-galactosidase is released from the bacteria to the external solid medium where it hydrolyzes X-Gal substrate analogue, developing a blue halo at the edge of the inhibition growth zone. The assay was successfully evaluated against several antibiotics with well-known mechanism of action. It was found that bacteriostatic compounds consistently did not display blue halo at the inhibition zone. (C) 2000 Elsevier Science B.V. All rights reserved.

Más información

Título según WOS: Chromogenic plate assay distinguishing bacteriolytic from bacteriostatic activity of an antibiotic agent
Título según SCOPUS: Chromogenic plate assay distinguishing bacteriolytic from bacteriostatic activity of an antibiotic agent
Título de la Revista: JOURNAL OF MICROBIOLOGICAL METHODS
Volumen: 40
Número: 3
Editorial: ELSEVIER SCIENCE BV
Fecha de publicación: 2000
Página de inicio: 199
Página final: 206
Idioma: English
URL: http://linkinghub.elsevier.com/retrieve/pii/S0167701200001251
DOI:

10.1016/S0167-7012(00)00125-1

Notas: ISI, SCOPUS