Genistein and curcumin block TGF-beta(1)-induced u-PA expression and migratory and invasive phenotype in mouse epidermal keratinocytes

Santibanez, JF; Quintanilla, M; Martinez, J.

Abstract

Transforming growth factor-beta(1) (TGF-beta(1)) stimulates migration/invasion of mouse transformed keratinocytes and increases urokinase (u-PA) expression/secretion. In this report, we analyzed the biological behavior of two naturally occurring inhibitors of protein tyrosine kinases, genistein and curcumin, that could abrogate the enhancement of u-PA levels induced by TGF-beta(1) in transformed keratinocytes. Our results showed that genistein and curcumin blocked this response in a dose-dependent manner and also inhibited the TGF-beta(1)-induced synthesis of fibronectin, an early responsive gene to the growth factor. Both compounds also reduced TCF-beta(1)-stimulated cell migration and invasiveness. These results suggest that a tyrosine kinase-signaling pathway should be involved in TGF-beta(1)-mediated increased malignancy of transformed keratinocytes and that genistein and curcumin could play an important role in inhibiting tumor progression.

Más información

Título según WOS: Genistein and curcumin block TGF-beta(1)-induced u-PA expression and migratory and invasive phenotype in mouse epidermal keratinocytes
Título de la Revista: NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL
Volumen: 37
Número: 1
Editorial: ROUTLEDGE JOURNALS, TAYLOR & FRANCIS LTD
Fecha de publicación: 2000
Página de inicio: 49
Página final: 54
Idioma: English
URL: http://www.tandfonline.com/doi/abs/10.1207/S15327914NC3701_6
DOI:

10.1207/S15327914NC3701_6

Notas: ISI