Cloning, cellular distribution and functional expression of small intestinal epithelium guinea pig C1C-5 chloride channel

Cornejo I.; Niemeyer, MI; Sepulveda, FV; Cid, LP

Abstract

We report the cloning of a guinea pig ClC-5 chloride channel (gpClC-5) from distal small intestinal epithelial cells by RT-PCR and RACE. The transcript is shown to be present in duodenum, jejunum and ileum epithelium by RT-PCR and Northern analysis. This is confirmed by in situ hybridisation which also shows the transcript to be homogeneously distributed in the crypt and villus regions. Expression of gpClC-5 in HEK-293 cells generated markedly outwardly rectified chloride currents with a perm-selectivity sequence of NO3- > Cl- > Br- > I- > F- > gluconate-. The possible role of gpClC-5 in this epithelial location is discussed. © 2001 Elsevier Science B.V.

Más información

Título según WOS: Cloning, cellular distribution and functional expression of small intestinal epithelium guinea pig C1C-5 chloride channel
Título según SCOPUS: Cloning, cellular distribution and functional expression of small intestinal epithelium guinea pig ClC-5 chloride channel
Título de la Revista: BBA – Biomembranes
Volumen: 1512
Número: 2
Editorial: ELSEVIER SCIENCE BV
Fecha de publicación: 2001
Página de inicio: 367
Página final: 374
Idioma: English
URL: http://linkinghub.elsevier.com/retrieve/pii/S0005273601003315
DOI:

10.1016/S0005-2736(01)00331-5

Notas: ISI, SCOPUS