Real-Time Quantitative Polymerase Chain Reaction (QPCR) for the Identification and Quantification of Strptococcus Mutans in Saliva and Dental Biofilm in Children.

Moncada G , Duperat LDC , Palma P , Corsini G , Neira M , Reyes E , Oliveira Junior OB , Faleiros S , Gordan V , Yévenes I

Keywords: saliva, qpcr, s. mutans, dental biofilm, gtfB gen


Introduction: the objective of this study was to use real-time qPCR to identify and quantify the Streptococcus mutans species in samples of saliva and dental biofilm. Methods: 27 children were randomly chosen with the following criteria: 8 years of age, low socio-economic levels, residing in the northern metropolitan area of Santiago de Chile; they were asked to attend an appointment while fasting with no teeth brushing for at least 12 hours, in order to collect non-stimulated saliva and a pool of supragingival dental biofilm of all the mesio-vestibular sides of anterior and posterior teeth. The amount of S. mutans in the samples was quantified by qPCR using primers that amplify a fragment of the gtfB gene of S. mutans. Results: the amplification showed 98% efficiency with a fluorescence of 3.36 cycles. The melting curve presented a single maximum at the same temperature for all samples. Conclusion: the methodology allows the specific identification and quantification of gene gtfB of S. mutans in saliva and dental biofilm in a quick and reliable manner, contributing to the identification of individual cariogenic risk.

Más información

Título de la Revista: Rev Fac Odontol Univ Antioq
Volumen: 28
Número: 1
Fecha de publicación: 2016
Página de inicio: 71
Página final: 94
Idioma: english
Financiamiento/Sponsor: Appointed to Fonis Project SAE 13i20205